辅助T细胞表现出一个动态和可逆的3'-UTR景观
Denis Seyres1,2, Oliver Gorka3,2, Ralf Schmidt4
1Department of Biomedicine, Basel University Hospital and University of Basel, CH-4031 Basel, Switzerland denis.seyres@unibas.ch lukas.jeker@unibas.ch.
概括
在T细胞中,替代性多氨基化 (APA) 在免疫反应过程中动态改变3'未翻译区域 (3' UTRs). 这些变化主要影响RNA结合蛋白和microRNA相互作用,是短暂的,不会显著影响基因表达水平.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 在RNA生物学,RNA生物学.
背景情况:
- 3' 未翻译区域 (3' UTRs) 通过RNA结合蛋白 (RBP) 和微RNA相互作用来调节mRNA命运.
- 激活后,T细胞表现出动态的3' UTR修饰,但它们的功能意义尚不清楚.
研究的目的:
- 在T辅助细胞中研究取决于分裂的替代多基化 (APA).
- 确定3' UTR动态对T细胞激活和扩张过程中的基因表达和RNA调节的影响.
主要方法:
- 从各种CD4+T细胞子集 (天真,激活,记忆,调控) 中生成3'端测序数据.
- 估计3' UTR长度变化使用非负矩阵分解和PacBio长读序列.
- 分析了大量的RNA-seq数据,以评估APA和基因表达之间的关联.
主要成果:
- 发现T细胞中的APA事件是短暂的,在效应器阶段扩张后会逆转.
- 在APA和差异基因表达或转录使用之间没有观察到显著的关联.
- 对T细胞相关的微RNA和RBP的保留结合点在替代的3' UTR中被确定.
结论:
- 在T细胞中,替代性多基化在调节总体转录丰度方面起到边缘作用.
- 3' UTR 序列的修改,特别是 RBP 和 microRNA 结合点,对于控制 T 细胞命运和恒温至关重要.
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