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相关概念视频

X-ray Crystallography02:18

X-ray Crystallography

23.9K
The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
23.9K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.4K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.4K
X-ray Diffraction of Biological Samples01:10

X-ray Diffraction of Biological Samples

3.8K
X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...
3.8K

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相关实验视频

Updated: Jul 4, 2025

Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene
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来自单个X射线自由电子激光脉冲的3D原子结构.

Gábor Bortel1, Miklós Tegze2, Marcin Sikorski3

  • 1Wigner Research Centre for Physics, Institute for Solid State Physics and Optics, P.O.B. 49, 1525, Budapest, Hungary. bortel.gabor@wigner.hu.

Nature communications
|February 1, 2024
PubMed
概括

研究人员使用单个X射线自由电子激光 (XFEL) 脉冲确定了3D原子结构. 这一突破捕捉了快速的,不可重复的过程,达到X射线结构解决方案的时间极限.

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An Experimental Protocol for Femtosecond NIR/UV - XUV Pump-Probe Experiments with Free-Electron Lasers
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Sample Preparation and Transfer Protocol for In-Vacuum Long-Wavelength Crystallography on Beamline I23 at Diamond Light Source
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Sample Preparation and Transfer Protocol for In-Vacuum Long-Wavelength Crystallography on Beamline I23 at Diamond Light Source

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相关实验视频

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Sample Preparation and Transfer Protocol for In-Vacuum Long-Wavelength Crystallography on Beamline I23 at Diamond Light Source
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科学领域:

  • 材料科学 材料科学 材料科学
  • 晶体学 晶体学是指结晶学.
  • 物理 物理学 物理

背景情况:

  • X射线自由电子激光器 (XFEL) 提供独特的脉冲X射线功能.
  • 目前的方法缺乏从单个XFEL脉冲开始的原子结构确定.

研究的目的:

  • 为了证明从单个XFEL脉冲中3D原子结构的确定.
  • 为了确定X射线结构解决方案的新时间极限.

主要方法:

  • 使用单个25 fs XFEL脉冲的3D原子结构的实验性确定.
  • 通过GaAs和GaP的Kossel线图案对数百个布拉格反射进行并行测量.

主要成果:

  • 从单个XFEL脉冲中成功确定了3D原子结构.
  • 实现了迄今为止X射线结构解决方案的最终时间极限.

结论:

  • 这种方法可以在不可重复的快速过程中捕获晶体结构.
  • 开辟了研究极端条件下的物质和短暂结构的可能性.
  • 有助于显著更短的时间解决的探头结构研究.