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相关概念视频

RNA Editing02:23

RNA Editing

9.0K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.0K
Ribosome Profiling02:24

Ribosome Profiling

3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

10.6K
The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
10.6K
What is Gene Expression?01:36

What is Gene Expression?

8.5K
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
8.5K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

910
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
910
Leaky Scanning02:28

Leaky Scanning

5.1K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.1K

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相关实验视频

Updated: Jul 4, 2025

A Nonsequencing Approach for the Rapid Detection of RNA Editing
08:50

A Nonsequencing Approach for the Rapid Detection of RNA Editing

Published on: April 21, 2022

2.6K

基于RNA编辑介导的遗传密码扩展的追踪内源蛋白质.

Min Hao1, Xinyu Ling1,2, Yi Sun1

  • 1State Key Laboratory of Natural and Biomimetic Drugs, Chemical Biology Center, Department of Molecular and Cellular Pharmacology, Pharmaceutical Sciences, Peking University, Beijing, China.

Nature chemical biology
|February 1, 2024
PubMed
概括

我们开发了RNA编辑介导的非正规氨基酸蛋白质标记 (RENAPT) 用于活细胞内源性蛋白质的特定位点标记. 这种方法允许临时的,不可遗传的蛋白质标记,而无需对protospacer相邻动机序列的限制.

更多相关视频

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
07:46

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

5.8K
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

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相关实验视频

Last Updated: Jul 4, 2025

A Nonsequencing Approach for the Rapid Detection of RNA Editing
08:50

A Nonsequencing Approach for the Rapid Detection of RNA Editing

Published on: April 21, 2022

2.6K
CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
07:46

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

5.8K
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

33.9K

科学领域:

  • 分子生物学分子生物学
  • 细胞生物学 细胞生物学
  • 生物化学 生化学

背景情况:

  • 过度表达的文物限制了传统的蛋白质标记方法.
  • 基因组编辑使内源蛋白标记成为可能,但存在局限性.
  • 特定地点的标签对于研究蛋白质定位和功能至关重要.

研究的目的:

  • 建立一种新的方法,用于在活细胞中对特定区域内源性蛋白质进行标记.
  • 为了克服现有的蛋白质标记技术的局限性,例如过度表达和原空间体相邻动机依赖.
  • 为了实现内源蛋白质的实时成像和功能研究.

主要方法:

  • 开发RNA编辑介导的非正规氨基酸蛋白质标记 (RENAPT).
  • 使用光或生物直角非正规氨基酸进行标签.
  • 在包括初级神经元在内的多种细胞类型中应用.

主要成果:

  • RENAPT 能够对内源性蛋白质进行临时的,非遗传的,特定地点的标记.
  • 该方法独立于原空间体相邻的动图序列.
  • 在特定的亚细胞位置成功成像各种内源蛋白质.
  • 使用不同的非正规氨基酸同时标记两个不同的蛋白质.
  • 在主要神经元中实时标记离子通道和神经元特异性蛋白质.

结论:

  • RENAPT是一个多功能和广泛适用的平台,用于活细胞内源性蛋白质标记.
  • 这种技术有助于研究蛋白质的局部化和功能,没有过度表达的文物.
  • RENAPT为先进的细胞和分子研究提供了一个有前途的方法.