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在结直肠癌中miR-196a-1的诊断潜力

Bayan Mehrjoei¹, Lida Haghnazari¹, Homayoon Bashiri²

¹Department of Clinical Biochemistry, Kermanshah University of Medical Sciences, Kermanshah, Iran.

|February 2, 2024

概括

微RNA-196a-1 (miR-196a-1) 在结直肠癌 (CRC) 组织和血中显著上调,显示出作为CRC检测的非侵入性生物标志物的潜力.

科学领域:

分子生物学分子生物学
在瘤学瘤学.
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

大肠直肠癌 (CRC) 是一种普遍存在的全球性恶性瘤.
微RNAs (miRNAs) 是关键的表观遗传调节者,参与癌症的发展.
miR-196a-1在CRC病原体中的作用需要进行调查.

研究的目的:

评估CRC组织和血中的miR-196a-1表达.
评估homeobox D8 (HOXD8) 作为一个潜在的目标基因.
为了确定miR-196a-1作为CRC的生物标志物的诊断值.

主要方法:

使用定量逆转录聚合酶链反应 (qRT-PCR) 来测量miR-196a-1和HOXD8的表达.
样本包括来自55名CRC患者和55名健康对照的220个血和组织样本.
接收器操作特征 (ROC) 分析将miR-196a-1的诊断准确性与已确定的瘤标志物进行比较.

主要成果:

与对照组相比,miR-196a-1水平在CRC组织 (11.4倍) 和血 (11.7倍) 均显著升高 (p < 0.001).
ROC分析显示了miR-196a-1的高诊断性能,组织的曲线下面积 (AUC) 为0.925 (89%灵敏度,98%特异性) 和血的0.801 (70%灵敏度,98%特异性).
瘤组织中HOXD8表达没有显著的变化.

结论:

miR-196a-1表现出致癌性质,并在CRC发育中发挥作用.
miR-196a-1是一种有前途的新型非侵入性生物标志物,用于CRC检测.

关键词:

生物标志物生物标志物结肠直肠癌是一种癌症.在HOXD8基因中,分子诊断学分子诊断学在 miR-196a-1-1 中.

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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...