在阿布扎比 (阿联) 的家中发现kdr突变和德尔塔美林耐药性
Mohamad Hamdan1, Tamilarasan Kamalanathan2, Asim Iqbal2
1Biology Department, UAE University, P.O. Box 15551, Al Ain, UAE.
Parasites & vectors
|February 2, 2024
概括
阿布扎比的家携带抗击耐药性 (kdr) 突变,包括两种新突变,影响了甲状腺杀虫剂的有效性. 通过CDC瓶子生物测试证实了德尔塔美林耐药性,突出显示了更新控制策略的必要性.
科学领域:
- 昆虫学 昆虫学是一门学科.
- 分子生物学分子生物学
- 公共卫生 公共卫生
背景情况:
- 室内 (Musca domestica) 是重要的疾病载体.
- 甲状腺杀虫剂的使用通过电压敏感通道 (VSSC) 中的突变驱动耐药性.
研究的目的:
- 用分子方法识别阿联家中的抗击抗性 (kdr) 突变.
- 建立一个CDC瓶子生物试验,用于对阿联的家里进行德尔塔米特林耐药性查.
主要方法:
- 从阿布扎比,阿联的19个地点收集了成年人家中的.
- 提取了DNA并使用了特定基因的PCR放大 (PASA),常规PCR和桑格测序来分析VSSC基因区域.
- 设计了RT-PCR原料来检测kdr突变,并进行了CDC瓶式生物测试以检测德尔塔米特林耐药性.
主要成果:
- 在19个位置中,kdr基因基因存在于18个位置,发现了耐药的同卵性 (RR) 和异卵性 (RS) 基因型.
- 证实了已知的L1014F kdr突变,并在阿联发现了两个新的突变 (L1014H,T929I).
- 美国疾病预防控制中心 (CDC) 的瓶式生物试验检测到16个地点中的2个地点的家中存在deltamethrin耐药性 (12.5%的耐药性).
结论:
- 在阿布扎比的家里中确认已知和发现了新的kdr突变.
- 在阿联的家种群中检测到德尔塔米特林耐药性.
- 建议进行进一步的研究,以确定更多的kdr突变,并为杀虫剂控制策略提供信息.
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