深色基因编辑触发了Ceratitis capitata中对温度敏感的致命表型.
Germano Sollazzo1,2,3, Katerina Nikolouli1, Georgia Gouvi1,4,3
1Insect Pest Control Laboratory, Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, Friedensstrasse 1, Seibersdorf, 2444, Austria.
BMC biotechnology
|February 2, 2024
概括
研究人员确定了地中海果中的深色基因是开发温度敏感致命 (tsl) 特性的候选者. 基因编辑成功地创造了TSL突变菌株,为在害虫控制中创建基因性别定性菌株 (GSS) 提供了一个新的工具.
科学领域:
- 昆虫学 昆虫学是一门学科.
- 遗传学 遗传学 是一个
- 虫害管理 虫害管理 虫害管理
背景情况:
- 地中海果 (Ceratitis capitata) 是一个主要的农业害虫.
- 全面综合害虫管理 (AW-IPM) 和无菌昆虫技术 (SIT) 是关键的控制策略.
- 基因性别定性菌株 (GSS) 通过允许仅限男性的释放来提高SIT效率.
研究的目的:
- 为了研究深色 (Ccdor) 基因作为温度敏感致命 (tsl) 现型的候选者.
- 开发一种使用可选择标记器构建GSS的通用方法.
- 为了识别和描述 GSS 开发中的 Ccdor 中的突变.
主要方法:
- 细胞遗传学,基因组学和生物信息学被用来定位和描述Ccdor基因.
- 使用CRISPR/Cas9基因编辑 (NHEJ和HDR) 来诱导Ccdor.中的突变.
- 突变菌株在不同温度下对TSL表型和健康成本进行了评估.
主要成果:
- 该Ccdor基因定位在假定TSL区域的5号染色体上.
- 在CRISPR/Cas9基因编辑中,Ccdor突变菌株表现出TSL表型.
- 开发的菌株在标准养殖温度 (25°C) 时表现出最小的健康成本,在高温 (36°C) 时表现出完全的致命性.
结论:
- 深色基因的基因编辑成功地产生了状炎中温度敏感的致命突变菌株.
- 这些发现表明,保存的深色基因是开发新型GSS的可行目标.
- 这种方法有可能在其他昆虫害虫和疾病载体中产生GSS.
相关概念视频
02:27Background and Environment Affect Phenotype
6.5K
Although the genetic makeup of an organism plays a major role in determining the phenotype, there are also several environmental factors, such as temperature, oxygen availability, presence of mutagens, that can alter an organism’s phenotype.
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
6.5K
02:41Lethal Alleles
15.4K
Agouti: A Lethal Allele
Lucien Cuénot discovered lethal alleles in 1905 while studying the inheritance of coat color in mice. The agouti gene is responsible for the color of the coat in mice. This gene codes for an agouti-signaling protein, which is responsible for melanin distribution in mammals. The wild-type allele gives rise to gray-brown coat color in mice, while the mutant allele gives rise to yellow coat color. In addition to coat color, the agouti gene is associated with the yellow...
Lucien Cuénot discovered lethal alleles in 1905 while studying the inheritance of coat color in mice. The agouti gene is responsible for the color of the coat in mice. This gene codes for an agouti-signaling protein, which is responsible for melanin distribution in mammals. The wild-type allele gives rise to gray-brown coat color in mice, while the mutant allele gives rise to yellow coat color. In addition to coat color, the agouti gene is associated with the yellow...
15.4K