tRFUniverse:对于人类癌症中tRNA衍生的ncRNA的交互分析的综合资源
Alessandro La Ferlita1, Salvatore Alaimo2, Giovanni Nigita1
1Department of Cancer Biology and Genetics, The James Comprehensive Cancer Center, The Ohio State University, Columbus, OH, USA.
iScience
|February 2, 2024
概括
来自tRNA的ncRNAs调节基因表达,并与癌症有关. 新的tRFUniverse资源提供了对人类癌症中这些非编码RNA的全面分析,有助于研究.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 癌症研究 癌症研究
背景情况:
- 来自tRNA的非编码RNA (ncRNA) 是基因表达的新兴调节者.
- 这些ncRNA越来越多地与各种疾病,特别是癌症有关.
- 对于tRNA衍生的ncRNAs的现有在线资源缺乏全面的分析功能.
研究的目的:
- 介绍tRFUniverse,这是一个新的在线资源,用于分析人类癌症中tRNA衍生的ncRNA.
- 在多种癌症数据集中提供广泛的tRNA衍生的ncRNAs集合.
- 整合分子相互作用数据,以更深入地了解它们在癌症中的作用.
主要方法:
- 在TCGA和TARGET瘤队列,NCI-60细胞系和生物流体中分析tRNA衍生的ncRNA.
- 使用公开的AGO CLASH/CLIP-Seq数据来识别RNA-RNA相互作用.
- 将各种数据集集集成到一个统一的在线平台中.
主要成果:
- tRFUniverse提供了人类癌症中tRNA衍生的ncRNA的全面目录.
- 该资源包括跨多种癌症类型和样本来源的分析.
- 通过集成的奥米克数据,确定了涉及tRNA衍生的ncRNA的分子相互作用.
结论:
- tRFUniverse为癌症研究提供了一个有价值的综合平台.
- 促进对tRNA衍生的ncRNAs在癌症生物学中的作用的研究.
- 增强在瘤发生过程中对非编码RNA功能的研究.
相关概念视频
02:55The Nucleolus
8.8K
The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
8.8K
02:39lncRNA - Long Non-coding RNAs
8.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.6K
03:18Non-LTR Retrotransposons
11.5K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
11.5K
03:21RNA-seq
10.0K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.0K