氧化合成酶引导的基因组挖掘确定了一种用于细菌特殊代谢中的烯酸化酶的细胞染色体P450酶
Hu Li1, Wei Li2, Kaihui Song2
1Polytechnic Institute, Zhejiang University, Hangzhou, 310022, China.
Synthetic and systems biotechnology
|February 2, 2024
概括
研究人员发现了一种新的抗生素,拉约拉米,使用一种由氧化合成酶 (NOS) 引导的基因组挖掘策略. 他们发现了一种新型的细胞染色体P450酶,该酶在天然产品生物合成中催化烯酸.
科学领域:
- 生物化学 生物化学
- 微生物学 微生物学
- 自然产品化学 自然产品化学
背景情况:
- 氧化 (NO) 是一种生物信号分子,越来越多地被认为是微生物天然产品生物合成的前体.
- 参与NO生产途径的基因在这些化合物的生物合成基因集群 (BGC) 中被发现.
- 许多NO衍生的天然产品的生物合成仍然不太了解.
研究的目的:
- 通过使用氧化合成酶 (NOS) 引导的基因组挖掘方法,发现NO衍生细菌天然产品和NO利用的生物催化剂.
- 为了阐明含有酸四烯的抗生素拉乔拉米的生物合成途径.
- 确定参与天然产品生物合成的新型酶,特别是那些利用NO.
主要方法:
- 基因组采矿使用在动态细菌基因组中发现的保存的含NOS的BCG.
- 在体内和体外生化分析.
- 酶活性的表征,重点关注酸的化.
主要成果:
- 鉴定和表征一种保存的含有NOS的BGC,该BGC是负责拉约拉米生物合成的.
- 发现了第一个能够在天然产品生物合成中催化油酸的细胞染色体P450酶.
- 展示一种有效的策略,用于识别使用NO的代谢途径和新型化生物催化剂.
结论:
- 该研究扩大了对天然产品化学中的生物合成化过程的理解.
- 已经确定了一种新型的细胞染色体P450酶,进步生物催化剂领域.
- 通过NOS引导的基因组挖掘策略为发现新的NO衍生天然产品和相关酶提供了强大的工具.
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