在骨质疏松性缺陷中,FGF2/HGF原始化有助于脂肪衍生干细胞介导的骨形成
Jeong Seop Park1, Do Young Kim1, Hyun Sook Hong1,2,3
1Department of Biomedical Science and Technology, Graduate School, Kyung Hee University, Seoul, 02447, South Korea.
Heliyon
|February 2, 2024
概括
用纤维细胞生长因子2 (FGF2) 和肝细胞生长因子 (HGF) 进行脂肪衍生干细胞 (ADSC) 的原始化,可以增强其骨质生成潜力. 这种方法改善了骨质疏松性缺陷中的骨再生,为骨疾病治疗提供了一个有前途的策略.
科学领域:
- 干细胞生物学和再生医学.
- 生物材料和组织工程用于骨再生.
背景情况:
- 脂肪源干细胞 (ADSCs) 的活性因供体年龄和疾病而受损,影响细胞治疗的疗效.
- 骨质疏松症会对ADSC活力和骨质分化产生负面影响,通常与FGF2和HGF信号缺陷有关.
研究的目的:
- 在骨质疏松症模型中研究FGF2和HGF补充对ADSC介导骨质生成的影响.
- 在骨质疏松症条件下探索FGF2/HGF对ADSCs作用的潜在机制.
主要方法:
- ADSCs从卵巢切除 (OVX) 的老鼠中分离出来,并用FGF2和HGF在骨质基质中培养.
- 用原始的ADSC被自主移植到OVX大鼠大腿部缺陷中,使用酸酸基架.
- 移植后四周评估了骨的形成.
主要成果:
- FGF2/HGF原始化促进了OVXADSCs在体外未成熟的骨质母细胞的形成,提高了关键的骨质生殖标志物 (Runx-2, osterix,ALP) 的调节.
- 在骨质诱导过程中,原始化增加了ADSC微环境中的VEGF和SDF-1α水平.
- 移植FGF2/HGF原料的OVXADSCs显著增强了骨再生,由1型原蛋白和骨质素表达证实.
结论:
- 将ADSCs与FGF2和HGF进行原始化,可以提高它们的分化潜力和治疗疗效.
- 这一策略在老年骨疾病中具有自身干细胞治疗的潜力.
相关概念视频
01:40Bone Remodeling
Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
01:31Bone Remodeling and Repair
Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during bone...