相关实验视频

Updated: Jul 4, 2025

Introducing a Gene Knockout Directly Into the Amastigote Stage of Trypanosoma cruzi Using the CRISPR/Cas9 System
06:13

Introducing a Gene Knockout Directly Into the Amastigote Stage of Trypanosoma cruzi Using the CRISPR/Cas9 System

Published on: July 31, 2019

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一种基于2A的多功能策略,用于Trypanosoma cruzi的子宫外表达和内源基因标记

Gabriela T Niemirowicz1, Giannina Carlevaro1, Oscar Campetella1

  • 1Instituto de Investigaciones Biotecnológicas, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Universidad Nacional de San Martín-Escuela de Bio y Nanotecnologías (EByN). Campus Miguelete, 25 de Mayo y Francia (B1650HMP), San Martín, Argentina.

Heliyon
|February 2, 2024
PubMed
概括

研究人员使用自切割2A开发了新的Trypanosoma cruzi表达载体. 这一策略增强了转基因表达,并创造了更加均的寄生虫种群,提高了研究效率.

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High-throughput Gene Tagging in Trypanosoma brucei

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In Vitro Drug Screening Against All Life Cycle Stages of Trypanosoma cruzi Using Parasites Expressing β-galactosidase

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相关概念视频

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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