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在低温下分析固定冷适应ANAMMOX颗粒的除性能和冷适应机制

Lin-Hua Zhang^1,2, Jing Zhang², Xiang Hu¹

¹College of Chemical Engineering, Beijing University of Chemical Technology, Beijing, China.

Water environment research : a research publication of the Water Environment Federation

|February 2, 2024

概括

固定冷适应颗粒在无氧氧化 (ANAMMOX) 过程中显著改善了低温去除. 这种增强是由于生物质保留和微生物聚合更好,有助于寒冷适应.

科学领域:

环境微生物学环境微生物学
废水处理技术废水处理技术
生物地质化学循环是什么

背景情况:

低温阻碍了无氧氨氧化 (ANAMMOX) 过程的效率,这些过程对去除至关重要.
开发适用于寒冷环境的强大的ANAMMOX系统对于有效的废水处理至关重要.

研究的目的:

为了提高ANAMMOX在低温下的性能,使用固定冷适应颗粒.
分析这些颗粒的低温除能力和冷适应机制.

主要方法:

固定冷适应ANAMMOX颗粒的制备 (R3) 和与非固定 (R2) 和普通颗粒 (R1) 的比较.
评估在各种低温 (5-18°C) 上的全无机 (TIN) 清除效率.
对生物质保留,细胞外聚合物 (EPS) 含量,ANAMMOX活性,微生物群体结构和冷应激蛋白质表达的分析.

主要成果:

与R2和R1.1.相比,固定颗粒 (R3) 显示出明显更高的TIN去除效率 (在11±2°C时高达68%,在7±2°C时高达54%)
R3表现出优越的生物质保留 (4.3-4.9 mg/gVSS) 和更高的紧密结合EPS (TB-EPS) 蛋白质含量,促进微生物聚合.
在5-25°C的R3中,ANAMMOX活性和heme c含量仍然较高,在较低的温度下,主导属 (Candidatus Kuenenia到Candidatus Brocadia) 的变化,以及冷压蛋白的活性表达.

结论:

冷适应ANAMMOX颗粒的固定有效地提高了冷废水处理中的去除效率.
固定化策略增强了生物质保留和微生物聚合,这对于在低温下保持ANAMMOX活动至关重要.
亚纳莫克斯属通过调节寒冷压力蛋白质表达来适应寒冷条件,固定化促进了这种适应反应.

关键词:

一个安纳姆莫克斯 (ANAMMOX).寒冷压力蛋白质的作用.细胞外聚合物质是一种细胞外聚合物质.固定化固定化固定化低温低温的低温是一个问题.

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