甲沙辛酸乙子的依赖于酸盐的生长
Marcus Richter1, Christian Sattler1, Christian Schöne1
1Fakultät Biologie, Technische Universität Dresden, Dresden, Germany.
Journal of bacteriology
|February 2, 2024
概括
海洋甲原体Methanosarcina acetivorans生长在pyruvate上,这是一种以前仅限于淡水物种的基质. 这表明甲原性古生物具有更广泛的异质性能力,独立于甲生产.
科学领域:
- 微生物学 微生物学
- 古代生物的新陈代谢
- 厌氧消化 厌氧消化
背景情况:
- 甲基生成对于无氧生物质降解至关重要,这种降解完全由甲基原始物进行.
- 甲基生物利用有限的基质范围,主要是乙和H2+CO2.
- 酸盐是关键的碳基质,但只有一种物种被认为可以利用它.
研究的目的:
- 为了研究海洋甲原体Methanosarcina acetivorans的pyruvate利用能力.
- 为了确定M. acetivorans是否可以使用pyruvate作为唯一的碳和能量来源生长.
- 了解所涉及的代谢途径和节能策略.
主要方法:
- 在定义的介质中培养M. acetivorans,其中pyruvate作为唯一的碳来源,有或没有CO2.
- 使用2-甲硫酸盐 (BES) 抑制甲基生成并评估替代代代谢途径.
- 分析代谢物概况以识别酸盐代谢的最终产品.
主要成果:
- 甲沙辛酸乙醇在酸盐上显示出强大的,CO2独立的生长.
- 增长速度表明活跃的pyruvate跨细胞质膜转移.
- 抑制BES的培养物呈现出乙烯基生长,表明pyruvate氧化和电子沉降通路.
结论:
- 甲沙酸是第二种在酸盐上生长的甲产物种,扩大了已知的基质范围.
- 酸盐的代谢在M. acetivorans不同于其他Methanosarcina物种,表明不同的异构能力.
- 在甲生成的古生物中,能量保存可以独立于甲形成,通过乙生成发生.
相关概念视频
01:20Fates of Pyruvate
8.5K
Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
8.5K
01:15Pyruvate Oxidation
159.0K
After glycolysis, the charged pyruvate molecules enter the mitochondria via active transport and undergo three enzymatic reactions. These reactions ensure that pyruvate can enter the next metabolic pathway so that energy stored in the pyruvate molecules can be harnessed by the cells.
First, the enzyme pyruvate dehydrogenase removes the carboxyl group from pyruvate and releases it as carbon dioxide. The stripped molecule is then oxidized and releases electrons, which are then picked up by NAD+...
First, the enzyme pyruvate dehydrogenase removes the carboxyl group from pyruvate and releases it as carbon dioxide. The stripped molecule is then oxidized and releases electrons, which are then picked up by NAD+...
159.0K