长非编码RNA H19X作为单核细胞对系统性硬化症内皮粘附的调节者
Francesca Tirelli1,2, Elena Pachera1, Sabrina Gmür1
1Center of Experimental Rheumatology, Department of Rheumatology, University Hospital Zurich, University of Zurich, Zurich, Switzerland.
Rheumatology (Oxford, England)
|February 2, 2024
概括
长非编码RNA H19X在硬化皮质 (SSc) 中被上调,并通过调节像VCAM1这样的粘附分子来促进内皮细胞激活,从而导致SSc血管病变.
科学领域:
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
- 血管生物学 血管生物学
背景情况:
- 硬化皮质 (SSc) 是一种系统性自身免疫性疾病,其特征是血管病变.
- 内皮细胞 (EC) 激活是SSc血管病变中的一个关键过程.
- 人们越来越认识到长非编码RNAs (lncRNAs) 在SSc病变发生中的作用.
研究的目的:
- 调查H19 X链接 (H19X) lncRNA在SSc.内皮细胞激活中的功能相关性.
- 确定H19X是否影响ECs中的粘附分子的表达.
主要方法:
- 在使用单细胞RNA测序 (scRNA-seq) 的SSc皮肤活检中分析H19X表达.
- 人体皮肤微血管EC (HDMEC) 的功能研究涉及H19X敲击.
- 通过qPCR和西方布洛特评估粘附分子水平 (例如VCAM1).
- 对内皮细胞粘附和VCAM1脱落的评估.
主要成果:
- 与健康对照人群相比,SSc患者的EC中H19X显著上调.
- 在HDMEC中,H19X Knockdown降低了多个粘附分子的表达,特别是VCAM1.1.
- H19X敲击降低了EC收缩性,PBMC对EC的粘附,以及VCAM1的脱落.
结论:
- lncRNA H19X在SSc血管病变中的EC激活中起作用.
- H19X作为EC中的粘附分子表达的调节者,可能有助于疾病的发病.
关键词:
这就是H19X.在VCAM1中使用VCAM1.激活的内皮质被激活的内皮质粘附分子的粘附分子干扰子干扰子干扰子长非编码RNA是什么意思微血管内皮细胞是什么?这就是scRNA-seqq.系统性硬化症 系统性硬化症血管病变是一种血管病变.更多相关视频
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