微空气对无氧污泥中合成和甲原活性的影响
Bruno P Morais1,2,3, Carla P Magalhães1,2, Gilberto Martins1,2
1CEB - Centre of Biological Engineering, University of Minho, Braga, Portugal.
Applied microbiology and biotechnology
|February 2, 2024
概括
微透气通过刺激有氧细菌来增强无氧消化,这些细菌保护敏感的无氧微生物. 这种相互作用对于在低氧水平的过程中具有弹性微生物群落的关键.
科学领域:
- 微生物学 微生物学
- 环境科学 环境科学
- 生物技术是生物技术.
背景情况:
- 微空气可以改善无氧消化 (AD),但氧会抑制像合成细菌和甲基生物等有约束力的无氧生物.
- 在微型有氧条件下了解微生物相互作用对于优化AD过程至关重要.
研究的目的:
- 研究微空气对合成菌群的影响及其与有氧细菌 (AB) 或选择性无氧细菌 (FAB) 的相互作用.
- 评估AB/FAB在微空气条件下维持功能性同位素联合体的保护作用.
主要方法:
- 用乙醇化无氧污泥并增加氧气度 (0-5%).
- 使用乙酸盐或H2/CO2作为甲基生物的直接基质进行测试.
- 监测基质消耗和甲生产率 (MPR).
主要成果:
- 氧气显著降低了酸盐或H2/CO2的基质消耗和MPR,在5%O2下抑制高达100%.
- 乙醇降解的显著抑制 (>36%) 仅在氧度超过2.5%时才发生.
- 氧气消耗表明乙醇对AB/FAB的刺激,从而保护了同位素群体.
结论:
- 选择性无氧细菌和有氧细菌在微型有氧条件下在维持功能和弹性合成菌群中发挥着至关重要的作用.
- 这些发现对于具有偶然氧气的常规AD系统和采用微空气化策略的AD系统具有重要意义.
- 微空气化研究对于优化利用受控氧气水平的AD系统至关重要.
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