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Updated: Jul 4, 2025

Benchtop Immobilized Metal Affinity Chromatography, Reconstitution and Assay of a Polyhistidine Tagged Metalloenzyme for the Undergraduate Laboratory
08:02

Benchtop Immobilized Metal Affinity Chromatography, Reconstitution and Assay of a Polyhistidine Tagged Metalloenzyme for the Undergraduate Laboratory

Published on: August 23, 2018

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Fus-SMO:化学烯单氧化酶的动力学,生物化学特征和在中建模,证明了定量合效率

Tanja Knaus1, Peter Macheroux2, Francesco G Mutti1

  • 1Van 't Hoff Institute for Molecular Sciences, HIMS-Biocat, University of Amsterdam, Science Park 904, Amsterdam, 1098 XH, The Netherlands.

Chembiochem : a European journal of chemical biology
|February 2, 2024
PubMed
概括

这项研究设计了一种聚烯单氧化酶 (Fus-SMO) 酶,揭示了增强的FADH2道化和改善的催化效率. 与自然系统相比,Fus-SMO表现出优越的性能,推动了生物催化.

关键词:
生物催化剂的生物催化剂辅助因素的道化亚黄素依赖的酶 - - 亚黄素依赖的酶聚变酶 聚变酶 聚变酶聚乙烯单氧化原酶是什么?

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Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition08:31

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

Published on: October 3, 2018

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Monitoring the Reductive and Oxidative Half-Reactions of a Flavin-Dependent Monooxygenase using Stopped-Flow Spectrophotometry12:08

Monitoring the Reductive and Oxidative Half-Reactions of a Flavin-Dependent Monooxygenase using Stopped-Flow Spectrophotometry

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