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在麦双胞胎连接 PI 258731 (Avena strigosa) 中映射冠状抗性

Rawnaq N Chowdhury¹, Tyler Gordon², Md Ali Babar³

¹Oak Ridge Institute for Science and Education (ORISE) Research Participant, Small Grains and Potato Germplasm Research Unit, Aberdeen, Idaho, United States of America.

|February 2, 2024

概括

研究人员在A. strigosa PI 258731.1.中发现了两种麦冠抗性基因. SNP标记物GMI_ES17_c6425_188在麦育种计划中显示了标记物辅助选择的前景.

科学领域:

植物病理学植物病理学
遗传学是一个遗传学.
农业学是一种农业学.

背景情况:

麦冠,是由Puccinia coronata f. sp. 引起的阿维纳 (Pca),显著限制了全球麦产量.
对Pca的耐药性已在二倍体麦物种中确定,特别是A. strigosa加入PI 258731,并成功地侵入了六倍体A. sativa.

研究的目的:

在PI 258731.中为皇冠抗的定量特征位置 (QTL) 地图.
确定A. strigosa的原始基因组位置与A. sativa的内向位置之间的关系.
确定Pca抗性的标记器辅助选择 (MAS) 的分子标记物.

主要方法:

从A. strigosa加入PI 258731和PI 573582.2之间的交叉中开发了168个F5:6重组杂交系 (RIL).
用6K Illumina SNP阵列对基因型RIL和父母进行基因定型,并对Pca分离物 (TTTG,QTRG) 的种苗反应进行评估.
进行了QTL分析的链接和间隔映射 (MIM),并开发了各种A. strigosa加入的SNP标记测试.

主要成果:

在LG7.7.上,两个距离0.6cm的阻力位点被映射到7.78cm的区域.
一个主要的QTL (QPc.FD-AS-AA4) 现场阻力被确定.
作为MAS的候选者,SNP标志物GMI_ES17_c6425_188与GMI_ES02_c37788_255.8一起被提议作为MAS的候选者.

结论:

这项研究成功地绘制了麦冠抗基,并确定了MAS的有希望的SNP标志物.
这项研究有助于利用PI 258731的耐药性在麦育种计划中用于对抗Pca.
开发的分子工具可以帮助检测和部署在各种麦胚胎质中的Pca耐药性.

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