一种广泛分布的细菌蛋白体隔离并储存元素硫
Robert Benisch1, Michael P Andreas2, Tobias W Giessen2
1Program in Chemical Biology, University of Michigan, Ann Arbor, MI 48109, USA.
Science advances
|February 2, 2024
概括
细菌封装剂在蛋白质外中储存元素硫晶. 这种封装增强了脱硫酶的活性,并保护硫免受细胞降解.
科学领域:
- 微生物学 微生物学
- 结构生物学 结构生物学
- 生物化学 生物化学
背景情况:
- 细菌利用基于蛋白质的区间进行储存和封存,这是由于缺乏膜结合的有机体.
- 囊素是广泛分布的 prokaryotic 蛋白质部分,参与各种细胞功能.
- 已知脱硫酶囊素与硫化合物相互作用.
研究的目的:
- 调查脱硫酶囊素的捕获和储存元素硫的能力.
- 阐明囊内硫储存的结构基础.
- 了解硫封装对脱硫酶活性和稳定性的功能影响.
主要方法:
- 低温电子显微镜 (cryo-EM) 用于在1.78-angstrom分辨率下确定含硫囊素的结构.
- 在体外实验中使用l-氨酸作为硫供体来诱导囊素内硫晶体的形成.
- 在具有不同硫源度的介质中培养细菌,以研究积累.
主要成果:
- 脱硫酶封装素有效地封存和储存大量的晶体元素硫.
- 1.78安格斯特罗姆的冷EM结构揭示了一个24纳米的脱硫酶载荷的囊外.
- 硫晶的形成取决于脱硫酶活性和l-氨酸的存在.
- 封装通过排除细胞减少剂来增强脱硫酶活性和稳定性.
结论:
- 脱硫酶囊素为 prokaryotes 提供了一种机制来储存可氧化还原性元素硫.
- 蛋白质外作为选择性透的屏障,保护储存的硫.
- 封装为脱硫酶酶提供了保护和稳定环境.
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