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(‒) - 甲基因通过激活编程细胞死亡途径,显示出对Acanthamoeba castellanii的氨基杀菌活性

Hương Giang Lê¹, Jung-Mi Kang¹, Tuấn Cường Võ¹

¹Department of Parasitology and Tropical Medicine, and Institute of Health Science, Gyeongsang National University College of Medicine, Jinju, 52727, Korea; Department of Convergence Medical Science, Gyeongsang National University, Jinju, 52727, Korea.

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概括

(-) - - 甲 (EC) 显示出有前途的抗阿梅巴活性,可以对抗Acanthamoeba castellanii. 这种天然化合物通过诱导编程细胞死亡来有效地杀死虫,为阿坎萨摩巴菌感染提供了潜在的新疗法.

科学领域:

微生物学微生物学
寄生虫学的寄生虫学
药理学药理学是指药理学的学科.

背景情况:

阿坎塔莫巴是一种机会性病原体,会引起人体严重的感染,如粒状阿米巴脑炎和阿坎塔莫巴角膜炎.
目前没有专门的药物可用于治疗Acanthamoeba感染,突出强调迫切需要新型治疗剂.
(-) -Epicatechin (EC),一种天然化合物,据报道表现出抗原生动物活性,使其成为药物开发的潜在候选者.

研究的目的:

为了评估 (-) - epicatechin (EC) 对 Acanthamoeba castellanii 的灭菌活性.
阐明EC的抗阿米巴作用背后的作用机制.
为了在体外评估EC的安全性和有效性,以对抗Acanthamoeba.

主要方法:

细胞活力测试用于确定EC对A. castellanii trophozoites的氨基杀菌活性及其对HCE-2和C6细胞的细胞毒性.
使用了亡/亡试验,TUNEL试验,线粒体功能障碍分析,caspase-3试验和qRT-PCR来研究作用机制.
此外,还评估了EC的囊杀菌活性.

主要成果:

欧共体对A.castelanii trophozoites (IC50 = 37.01 ± 3.96 μM) 显示出显著的氨杀菌活性,没有观察到对人类细胞系的细胞毒性.
在A. castellanii中,EC诱导了编程细胞死亡,其特征是DNA碎片化,活性氧物种增加,线粒体功能障碍和caspase-3的激活.
此外,EC还增加了自细胞形成和与自相关的基因的表达,部分杀死了囊,并抑制了excystation.

结论:

(-) - - 甲基因通过诱导编程细胞死亡,对Acanthamoeba castellanii trophozoites表现出强烈的氨杀菌活性.
欧盟委员会提出了一种有前途的治疗候选物或补充化合物,用于治疗阿坎萨摩巴菌感染.

关键词:

(‒) - 史诗形中华素.亚坎他莫埃巴 (Acanthamoeba) 是一种有毒的植物.它具有杀性活动.它具有囊杀菌活性.编程细胞死亡编程细胞死亡

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