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相关概念视频

Protein Networks02:26

Protein Networks

4.0K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
4.0K
Protein-protein Interfaces02:04

Protein-protein Interfaces

12.5K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
12.5K

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相关实验视频

Updated: Jul 4, 2025

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
13:56

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

Published on: July 18, 2013

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高吞吐量方法用于研究宿主-病原体相互作用期间的蛋白质-蛋白质相互作用.

Giridhar Chandrasekharan1, Meera Unnikrishnan1

  • 1Division of Biomedical Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom.

European journal of cell biology
|February 2, 2024
PubMed
概括

了解宿主-病原体蛋白-蛋白相互作用 (PPI) 对感染结果至关重要. 本综述强调了用于研究这些复杂的宿主-细菌PPI的高通量 (HT) 技术.

科学领域:

  • 微生物学 微生物学
  • 免疫学 免疫学 免疫学
  • 生物化学 生化学

背景情况:

  • 宿主-病原体蛋白-蛋白相互作用 (PPI) 是感染的关键决定因素.
  • 这些相互作用可以发生在宿主细胞内或外部,影响感染的严重程度.
  • 识别这些相互作用是了解病原体生存和宿主反应的关键.

研究的目的:

  • 审查主要的高通量 (HT) 基于实验室的技术,用于研究宿主细菌PPI.
  • 为了解决宿主-病原体相互作用的复杂性和多样性.
  • 提供对分析多个相互作用同时使用的公正方法的概述.

主要方法:

  • 对高通量 (HT) 技术现有文献的审查.
  • 专注于基于实验室的技术来进行宿主-细菌相互作用研究.
  • 包括生物化学,生物物理和生物信息学的方法.

主要成果:

  • 确定适用于宿主细菌PPI研究的几个关键高通量 (HT) 技术.
  • 讨论HT方法在处理宿主-病原体相互作用的复杂性方面的优势.
  • 强调这些查技术的公正性.
关键词:
高吞吐率的高吞吐量.宿主-病原体相互作用基于实验室的技术.质谱测量质量谱测量蛋白质与蛋白质的相互作用

更多相关视频

High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions
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High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

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Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions
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Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions

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相关实验视频

Last Updated: Jul 4, 2025

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
13:56

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

Published on: July 18, 2013

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High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions
14:58

High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

Published on: March 5, 2022

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Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions
05:37

Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions

Published on: October 20, 2020

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结论:

  • 高通量 (HT) 技术对于全面研究宿主-细菌蛋白-蛋白相互作用 (PPI) 是必不可少的.
  • 这些先进的方法有助于分析多种相互作用,有助于了解感染动态.
  • 审查的技术为未来对宿主-病原体相互作用研究提供了强大的工具.