相关实验视频

Updated: Jul 4, 2025

An Ex Vivo Tissue Culture Model for Fibrovascular Complications in Proliferative Diabetic Retinopathy

An Ex Vivo Tissue Culture Model for Fibrovascular Complications in Proliferative Diabetic Retinopathy

Published on: January 25, 2019

PI3Kδ通过患者衍生的玻璃体调解纤维化

Dan Liu¹, Bin Yan², Yiwei Yin³

¹Department of Ophthalmology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China; Department of Ophthalmology, Hunan Key Laboratory of Ophthalmology, Xiangya Hospital, Central South University, Changsha, Hunan, China.

Laboratory investigation; a journal of technical methods and pathology

|February 2, 2024

概括

来自黄斑膜患者的玻璃体通过PI3Kδ.驱动上皮层-介质细胞过渡 (EMT). 抑制PI3Kδ (氨基酸3-激酶-三角) 阻断了EMT和纤维化,这表明它是一种治疗膜 (ERM) 的治疗标.

科学领域:

眼科医生眼科眼科
细胞生物学细胞生物学
纤维化研究纤维化研究

背景情况:

表皮-介质细胞过渡 (EMT) 在纤维性疾病中至关重要,如表皮膜 (ERM),导致视力丧失.
来自增殖性糖尿病视网膜病变的玻璃体 (VIT) 通过Axl/PI3K/Akt通路刺激血管生成.

研究的目的:

为了研究来自黄斑膜患者的VIT对ARPE-19细胞的影响.
探索酸酸3-酶-三角酶 (PI3Kδ) 在ERM病原发生中的作用.

主要方法:

ARPE-19细胞被患者衍生的VIT治疗.
评估PI3Kδ功能使用p110δ淘汰和抑制剂idelalisib.
用激光诱导的纤维化模型来评估抗纤维化作用.

主要成果:

来自患者的VIT促进了ARPE-19细胞中的EMT和PI3Kδ表达.
p110δ绝杀/抑制减少了VIT诱导的EMT,细胞增殖和迁移.
在临床前模型中,Idelalisib治疗阻断了纤维化.

结论:

PI3Kδ (特别是p110δ) 对于ERM的进展至关重要.
准PI3Kδ是缓解视网膜纤维化的潜在治疗策略.
这项研究提高了对ERM机制和PI3Kδ的作用的理解.

关键词:

2019年1月19日 - - 时间皮质膜膜的皮质膜膜.表皮细胞-介质细胞过渡.有纤维化的纤维化.在p110δδ上

更多相关视频

Characterization of a Novel Human Organotypic Retinal Culture Technique

Characterization of a Novel Human Organotypic Retinal Culture Technique

Published on: June 9, 2021

Evisceration of Mouse Vitreous and Retina for Proteomic Analyses

Evisceration of Mouse Vitreous and Retina for Proteomic Analyses

Published on: April 3, 2011

相关概念视频

Introduction to Fibroblasts

Introduction to Fibroblasts

Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...