双性对男性化至关重要,但对Lygus hesperus的女性化不是必要的
J Joe Hull1, Chan C Heu1, Roni J Gross1
1USDA ARS, U.S. Arid Land Agricultural Research Center, Maricopa, AZ, 85138, USA.
Insect biochemistry and molecular biology
|February 2, 2024
概括
双性 (dsx) 基因对Lygus hesperusbug的雄性发育至关重要,但对雌性来说并不重要. 了解Lhdsx的功能有助于制定这种农业害虫的害虫防治策略.
科学领域:
- 昆虫遗传学 昆虫遗传学
- 发育生物学是发展生物学.
- 分子昆虫学分子昆虫学
背景情况:
- 昆虫的性别分化依赖于像双性 (dsx) 这样的转录因子.
- 与全体代谢昆虫相比,对半体代谢昆虫的性别确定知识有限.
- Lygus hesperus是一种重要的农业害虫,使其生物学与作物保护有关.
研究的目的:
- 在Lygus hesperus (Lhdsx) 中识别和描述双性 (dsx) 基因.
- 调查Lhdsx在这种半虫害虫的性别分化和发育中的作用.
- 探索这种知识在害虫管理中的潜在应用.
主要方法:
- 对Lhdsx.的全长转录识别和变体分析.
- 转录分析以确定表达模式.
- RNA干扰 (RNAi) 对于胚胎和幼虫阶段的基因淘汰.
- 对于淘汰赛研究的CRISPR/Cas9基因编辑.
主要成果:
- Lhdsx转录编码保存的蛋白质域,并无处不在表达,但性别偏差.
- RNAi和基因淘汰实验表明Lhdsx对于男性生殖器官和生殖器官发育至关重要.
- 女性发育不受Lhdsx操纵的影响,这表明独立的基底路径.
- 克里斯普尔/卡斯9导致雌性偏向的后代和男性发育受损.
结论:
- 在Lygus hesperus中,男性的发育需要Lhdsx,而女性的发育是dsx独立的.
- 这项研究揭示了一种独特的性别分化的模式,在一个半角虫害虫.
- 了解Lhdsx的功能为开发基于基因的新型害虫管理策略提供了基础.
相关概念视频
The Ratio of X Chromosome to Autosomes
8.5K
In most organisms, sex is determined by the ratio of X and Y chromosomes. However, in some organisms, such as Drosophila and C.elegans, sex is determined by the ratio of the number of X chromosomes to the number of sets of autosomes. The Y chromosome in Drosophila is active but does not determine sex. It contains genes responsible for the production of sperms in adult flies.
Normal male Drosophila has a ratio of one X chromosome to two sets of autosomes. In contrast, normal female...
Normal male Drosophila has a ratio of one X chromosome to two sets of autosomes. In contrast, normal female...
8.5K
Dosage Compensation
6.2K
In animals, gender is determined by the number and type of sex chromosome. For example, human females have two X chromosomes, and males have one X and one Y chromosome, whereas C.elegans with one X chromosome is a male, and the one with two X chromosomes is a hermaphrodite.
In addition to sexual development, the X chromosome has genes involved in autosomal functions such as brain development and the immune system. Therefore, males and females with distinct numbers of X chromosomes will...
In addition to sexual development, the X chromosome has genes involved in autosomal functions such as brain development and the immune system. Therefore, males and females with distinct numbers of X chromosomes will...
6.2K
The Y Chromosome Determines Maleness
6.6K
The Y chromosome is a sex chromosome found in several vertebrates and mammals, including humans. In addition to 22 pairs of autosomes, the human males have one X chromosome and one Y chromosome. In these organisms, the presence or absence of the Y chromosome determines the development of male traits.
Evolution
Around 300 million years ago, the two sex chromosomes diverged from two identical autosomal chromosomes. Over time, the Y chromosome has lost most of its genes, shrinking in size....
Evolution
Around 300 million years ago, the two sex chromosomes diverged from two identical autosomal chromosomes. Over time, the Y chromosome has lost most of its genes, shrinking in size....
6.6K
Background and Environment Affect Phenotype
6.5K
Although the genetic makeup of an organism plays a major role in determining the phenotype, there are also several environmental factors, such as temperature, oxygen availability, presence of mutagens, that can alter an organism’s phenotype.
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
6.5K
Development of the Sexual Organs in the Embryo and Fetus
720
Development of the reproductive organs in an embryo starts from a bipotential state. This means the early embryo can develop either male or female reproductive organs. The formation of these organs begins with the growth of gonadal ridges that arise from the intermediate mesoderm during the fifth week of development.
Near the gonadal ridges, two duct systems are present: the mesonephric ducts (Wolffian ducts) and paramesonephric ducts (Müllerian ducts). These ducts form the basis for the...
Near the gonadal ridges, two duct systems are present: the mesonephric ducts (Wolffian ducts) and paramesonephric ducts (Müllerian ducts). These ducts form the basis for the...
720
X-linked Traits
54.9K
In most mammalian species, females have two X sex chromosomes and males have an X and Y. As a result, mutations on the X chromosome in females may be masked by the presence of a normal allele on the second X. In contrast, a mutation on the X chromosome in males more often causes observable biological defects, as there is no normal X to compensate. Trait variations arising from mutations on the X chromosome are called “X-linked”.
54.9K


