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Wenjing Tang¹, Siqi Wu¹, Yin Tang¹

¹Department of Anesthesiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.

|February 2, 2024

概括

肥胖会通过增加IncFirre表达来加剧急性肺损伤 (ALI),从而促进炎症. 向IncFirre有效抑制炎症,为与肥胖相关的ALI提供了潜在的治疗策略.

科学领域:

分子生物学分子生物学
免疫学免疫学免疫学
遗传学遗传学是一个

背景情况:

肥胖与急性肺损伤/急性呼吸困扰综合征 (ALI/ARDS) 的炎症反应加剧有关.
长非编码RNAs (lncRNAs) 可能在肥胖对ALI的影响中发挥作用.
鉴定涉及的特定 lncRNA 对于理解疾病机制至关重要.

研究的目的:

为了调查 lncRNAs 在肥胖引起的 ALI 的参与.
为了确定潜在的效应因子 lncRNAs 导致肥胖相关的肺损伤.
探索已识别的 lncRNAs 在巨反应中的功能作用.

主要方法:

从正常,肥胖和肥胖的ALI小鼠的肺组织中分析lncRNA和mRNA的微阵列分析.
基因本体学 (GO) 和KEGG路径分析用于功能探索.
构建 lncRNA-mRNA 共表达网络,以识别关键的 lncRNA.
在人体样本中进行qRT-PCR验证,并在巨细胞中进行淘汰实验.
测量炎症媒介 (PGE2,NO) 和巨细胞极化.

主要成果:

肥胖在肺组织中显著改变了lncRNA和mRNA表达特征.
在肥胖小鼠和人类患者中,IncFirre表达升高.
lncFirre knockdown抑制了促炎媒介释放 (PGE2,NO) 和M1极化,同时在受到挑战的巨细胞中促进了M2极化.
lncFirre knockdown减轻了PA和LPS诱导的炎症和巨细胞两极分化.

结论:

lncFirre在与肥胖相关的ALI中受到上调,并导致肺炎.
干扰IncFirre有效地抑制了巨细胞的炎症.
lncFirre代表了一种与肥胖相关的ALI治疗的有希望的治疗标.

关键词:

急性肺损伤是什么?急性肺损伤是什么?炎症炎症是一种炎症.肥胖问题肥胖问题棕酸盐是一种棕酸盐.在第一个IncFirre.

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相关概念视频

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...