Pseudomonas aeruginosa 外膜囊泡包装的sRNA可以进入宿主细胞并调节先天免疫反应
Zhen Xie1, Xiao Wang1, Yangyang Huang2
1State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, 712100, China.
Pseudomonas aeruginosa 外膜囊泡 (OMVs) 提供细菌小RNA (sRNAs),以向宿主免疫基因. 这些OMV通过转移细菌sRNA来调节宿主天生的免疫力,揭示了一种新的宿主-病原体相互作用机制.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 细菌外膜囊泡 (OMVs) 通过传递毒性因子来调解宿主-病原体相互作用.
- OMV中的小RNA (sRNA) 可能会影响宿主mRNA的稳定性和功能.
研究的目的:
- 研究Pseudomonas aeruginosa OMVs在传递细菌sRNA调节宿主天生的免疫反应中的作用.
主要方法:
- 使用OptiPrep密度梯度超高速离心法,从P. aeruginosa中净化OMV.
- 通过小RNA-Seq.检测sRNA序列和丰度.
- 在分析 (miRanda,RNA混合) 和在RAW264.7细胞中的sRNA-宿主mRNA相互作用的实验验证.
主要成果:
- 在P. aeruginosa的OMV中确定了三种丰富的sRNA (sRNA4518698,sRNA2316613,sRNA809738) ,它们是非编码RNA的碎片.
- 证明这些sRNA在OMV中受到保护,并且可以向参与免疫反应的众多宿主mRNA.
- 表明合成sRNAs降低了宿主细胞内天生的免疫反应基因的表达.
结论:
- P. aeruginosa OMVs转移细菌sRNA,调节宿主天生的免疫力.
- 这种机制突出了通过OMVs通过sRNA传递对宿主反应的细菌调节的新途径.
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