相关实验视频
Updated: Jul 4, 2025

10:43
Modeling Oral-Esophageal Squamous Cell Carcinoma in 3D Organoids
Published on: December 23, 2022
3.4K
第二阶段食道癌:隐藏的危险与细胞重编程和由伪基因调节的瘤发生有关
Govada Pravallika1, Ramalingam Rajasekaran2
1Quantitative Biology Lab, Department of Integrative Biology, School of BioSciences and Technology, Vellore Institute of Technology, Vellore, Tamil Nadu, India.
BMC genomics
|February 2, 2024
概括
伪基因在食道癌中驱动细胞重编程和分化,特别是在第二阶段,影响瘤发生和患者预后. 它们的失调标志着一个独特的突变格局,有助于瘤的进展.
科学领域:
- 在瘤学瘤学.
- 基因组学就是基因组学.
- 分子生物学分子生物学
背景情况:
- 伪基因曾经被认为是非功能性的,现在越来越多地被认为是它们在细胞分化和发育中的调节作用.
- 伪基因在与癌症相关的分化,特别是食道癌中的特定参与仍然未得到充分研究.
- 这项研究调查了食道癌中的伪基因景观,以揭示它们在通过差异化事件促进瘤转化中的潜在作用.
研究的目的:
- 在食道癌中识别和表征与分化相关的伪基因.
- 阐明伪基因影响细胞重编程和瘤发生的调节机制.
- 建立伪基因表达模式作为食道癌的潜在预后和诊断生物标志物.
主要方法:
- 不同表达分析 (DeSeq2) 和网络分析 (InteractiVenn) 确定了关键的伪基因.
- 基因组丰富分析 (GSEA) 和ShinyGO进行了丰富分析.
- 贝叶斯网络分裂平均生成特定阶段的基因调节网络;使用MEME,STREME和Tomtom识别了转录因子和miRNA结合部位.
主要成果:
- 伪基因在第二阶段食道状细胞癌 (ESCA) 中表现出特定阶段的表达模式,与高细胞重编程,分化和性相关.
- 伪基因调节的基因 (SOX2,FEV,PRRX1,TFAP2A) 可能驱动细胞重编程和癌症发生在ESCAII阶段.
- 鉴定出APOBEC3A (A3A) 和APOBEC3G失调是第二阶段ESCA内重编程细胞中高突变的潜在驱动因素,具有明显的预后影响.
结论:
- 与分化相关的伪基因可以启动细胞重编程,促进第二阶段ESCA中的瘤转化.
- 假基因的组合失调作为高细胞分化和ESCAII阶段独特突变格局的标记.
- 伪基因表达模式和相关基因为食道癌提供了有价值的预后生物标志物.
相关概念视频
Cancer-Critical Genes I: Proto-oncogenes
8.9K
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
8.9K
Cancer-Critical Genes II: Tumor Suppressor Genes
7.4K
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
7.4K
Abnormal Proliferation
4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
Induced Pluripotent Stem Cells
4.1K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
Somatic...
4.1K
Rous Sarcoma Virus (RSV) and Cancer
5.1K
Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
5.1K
Cancers Originate from Somatic Mutations in a Single Cell
11.9K
Cancer arises from mutations in the critical genes that allow healthy cells to escape cell cycle regulation and acquire the ability to proliferate indefinitely. Though originating from a single mutation event in one of the originator cells, cancer progresses when the mutant cell lines continue to gain more and more mutations, and finally, become malignant. For example, chronic myelogenous leukemia (CML) develops initially as a non-lethal increase in white blood cells, which progressively...
11.9K

