唾液采集,运输,制备和储存方法的广泛比较:系统性审查
Hamed Mortazavi1, Amir-Ali Yousefi-Koma2,3, Hannaneh Yousefi-Koma4
1School of Dentistry, Shahid Beheshti University of Medical Sciences, Daneshjoo Blvd, Evin, Shahid Chamran Highway, Tehran, 1983963113, Iran.
BMC oral health
|February 2, 2024
概括
本系统性审查比较了人类唾液采集方法. 没有冷的即时分析对DNA是最好的,而特定的时间优化了生物标志物检测.
科学领域:
- 生物标志物的分析分析.
- 诊断流体利用的使用情况.
- 人的唾液研究研究.
背景情况:
- 人的唾液是一种非侵入性,可访问的诊断液体,可以替代血液.
- 关于唾液的收集,运输,制备和储存方法,没有先前的系统审查.
- 本综述解决了唾液分析标准化方案中的差距.
研究的目的:
- 系统地审查和比较各种人类唾液采样,处理,运输和储存技术的性能.
- 评估分析刺激或非刺激的人类唾液的方法.
- 为优化唾液样本完整性和生物标志物检测提供基于证据的建议.
主要方法:
- 按照PRISMA 2020指南进行的系统审查.
- 在Scopus,谷歌学者和PubMed数据库中执行电子文献搜索.
- 包括23项描述性人类临床研究 (1995-2022),调查了8种唾液特征/生物标志物和22种采样方法.
主要成果:
- 被动的唾液,Salivette®和吐是常见的采样方法.
- 鉴定了皮质醇 (7:30-9:00 AM), (10:30-11:00 AM) 和口腔癌代谢物 (2:00-8:00 PM) 的最佳采样时间.
- 没有离心/储存的即时分析优于DNA质量/数量;冷在-70°C至-80°C是常见的,但对DNA来说不太理想. 没有涂层的Salivette®最适合唾液流量.
结论:
- 结果为各种唾液收集,处理和储存方法提供了分类结果.
- 建议研究人员利用这些结果来为研究设计提供信息,并解决现有的方法差距.
- 唾液分析协议的标准化可以提高诊断准确性和研究可重复性.
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