相关实验视频

Updated: Jul 4, 2025

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

使用CRISPR-Cas9核糖核蛋白复合体编辑甲中的乙烯生物合成基因

Oluwaseun Suleimon Adedeji¹, Aung Htay Naing², Hyunhee Kang¹

¹Department of Horticulture, Kyungpook National University, Daegu, 41566, South Korea.

|February 2, 2024

概括

研究人员使用CRISPR/Cas9核糖核蛋白复合体精确编辑了花中的乙烯生物合成基因. 这种基因编辑技术通过准ACS1和ACO1.1等关键基因来改变植物特征,显示出有前途.

科学领域:

植物生物技术植物生物技术
分子生物学分子生物学
基因编辑基因编辑

背景情况:

乙烯 (ET) 生物合成对花的发育和收获后的生活至关重要.
针对ET生物合成基因,特别是1-aminocyclopropane-1-carboxylic acid (ACC) 合成酶1 (ACS1) 和ACC氧化酶1 (ACO1),提供了一个修改花特征的途径.
CRISPR/Cas9核糖蛋白 (RNP) 复合体为基因编辑提供了一个精确的工具.

研究的目的:

调查CRISPR/Cas9 RNP复合系统对编辑甲生物合成基因 (ACS1和ACO1) 的有效性.
在体外验证单指导RNAs (sgRNAs) 针对特定的目标基因分裂.
为了评估基因编辑效率和花原体和的内模式.

主要方法:

在 ACS1 和 ACO1 中对 sgRNA 设计的保护区域的验证.
在体外切割试验以确认sgRNA的特异性和有效性.
将sgRNA:Cas9 RNP复合体输送到花原生体中.
在原生质和衍生性中对目标基因的深度测序.

主要成果:

在体外测试证实了sgRNAs对ACS1和ACO1.1的高特异性和裂变效率.
在花原生体中进行基因编辑,导致ACO1的突变频率为8.810.8%,ACS1.0的突变频率为0.258.5%.
对的分析揭示了特定的内模式 (+1, -1, -8bp对于ACO1; -1, +1, +11bp对于ACS1).

结论:

该CRISPR/Cas9 RNP复合系统是有效的精确基因编辑乙烯生物合成基因在花.
sgRNAs在准ACS1和ACO1.1方面表现出高的特异性和效率.
这项研究证实了花在基因改造中的强大工具,这对特征改进有意义.

关键词:

这就是CRISPR/Cas9的作用.卡利卡利卡利卡利卡利乙烯生物合成基因乙烯生物合成基因在体外切割.印德尔图案印德尔图案印德尔图案印德尔图案原始塑料原始塑料是什么

更多相关视频

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner

Published on: July 1, 2021

相关概念视频

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

Homologous Recombination

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...