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TFEB通过促进线粒细胞和糖溶解之间的正反循环来调节牙纤维干细胞的形分化

Yiming Li¹, Enli Yang¹, Yiming Geng¹

¹School and hospital of Stomatology, Cheeloo College of Medicine, Shandong University, China; Department of Oral and Maxillofacial Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China.

Archives of oral biology

|February 3, 2024

概括

转录因子EB (TFEB) 促进牙髓干细胞 (DPSCs) 中的形细胞分化. 这一过程涉及菌和代谢重编程之间的反循环,这对骨再生至关重要.

科学领域:

干细胞生物学干细胞生物学
分子生物学分子生物学
再生医学是一种再生医学.

背景情况:

牙纸干细胞 (DPSC) 对于牙-纸复合物的再生至关重要.
转录因子EB (TFEB) 是溶酶体生物发生和自的关键调节者.
了解TFEB在DPSC分化中的作用对于开发新型再生疗法至关重要.

研究的目的:

为了研究TFEB对DPSCs. odontoblastic分化的调控作用.
阐明潜在的机制,包括细胞和代谢重编程.

主要方法:

用RNA测序来识别在差异化DPSC中差异表达的基因.
在体外测试 (CCK-8,EDU,ALP,ARS) 来评估细胞增殖和分化.
传输电子显微镜 (TEM) 和海马分析来检查细胞和细胞代谢.
在裸体小鼠体内进行皮下植入,以评估牙细胞分化.

主要成果:

过度表达TFEB显著增强了DPSC中的ALP活性和分化标志物,同时抑制了增殖.
在体内研究表明,TFEB促进骨形成,原沉积和DMP-1表达.
TFEB诱导了新陈代谢重编程和增加了线粒体,由线粒体自细胞和相关基因表达的增加证明.
糖解抑制剂 (2-DG) 和菌抑制剂 (Midiv-1) 抑制了TFEB诱导的DPSC分化,显示出一个正反循环.

结论:

在DPSCs中,TFEB充当了口腔芽细胞分化的积极调节者.
线粒和代谢重编程在TFEB介导的DPSC分化中形成了一个关键的正反循环.
这些发现凸显了TFEB作为增强牙纸再生的潜在治疗点.

关键词:

牙纸干细胞牙纸干细胞葡萄糖溶解是什么? 葡萄糖溶解线粒细胞衰变 (mitophagy) 是一种神经衰变的过程.体细胞质细胞.转录因子EB是EB的转录因子.

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