相关实验视频

Updated: Jul 4, 2025

Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana

Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana

Published on: May 21, 2019

由DGK5β衍生的酸通过稳定NADPH氧化酶来调节植物免疫中ROS的产生

Fan Qi¹, Jianwei Li¹, Yingfei Ai¹

¹Zhejiang Xianghu Laboratory, Department of Plant Protection, Zhejiang University, Hangzhou 310058, China.

Cell host & microbe

|February 3, 2024

概括

酸 (PA) 稳定植物免疫蛋白,增强反应性氧物种 (ROS) 的产生. 甲基甘油酶5 (DGK5) 产生PA,这对于植物对病原体的防御至关重要.

科学领域:

植物生物学植物生物学
分子植物病理学分子植物病理学
生物化学生化学

背景情况:

植物免疫力依赖于反应性氧物种 (ROS) 进行防御.
众所周知,酸 (PA) 通过与呼吸道爆发氧化酶同类D (RBOHD) 相互作用来调节ROS的产生.

研究的目的:

调查PA结合对RBOHD活动的影响.
阐明与RBOHD结合的PA生成机制.
了解二甲基甘油酶5 (DGK5) 在PA生产和植物免疫力中的作用.

主要方法:

分析PA对RBOHD蛋白稳定性和ROS产生的结合作用.
研究素诱导的ROS和PA的产生在二甲基甘激酶5 (DGK5) 突变.
使用DGK5异型 (DGK5α和DGK5β) 的补充研究.
局部定向突变发生,以分析DGK5β酸化在S506.6中的作用.

主要成果:

通过防止真空降解,PA结合增强了RBOHD蛋白的稳定性,从而导致基诱导的ROS产量增加.
在DGK5的突变损害了基诱导的PA和ROS产量.
DGK5β,但不是DGK5α,补充了PA和ROS生产中的缺陷,并恢复了dgk5-1突变中的Botrytis cinerea的耐药性.
在S506中DGK5β的酸化对其激活和PA的产生至关重要.

结论:

源自DGK5β的PA通过抑制RBOHD蛋白降解来调节ROS的产生,从而增强植物的免疫反应.
PA和ROS之间的相互作用是植物免疫信号的关键机制.
通过PA介导的RBOHD控制,DGK5β是植物免疫的关键调节者.

关键词:

在ROS生产生产中,ROS产量奇丁丁是一种.甲基甘油氨基基酶5 甲基甘油氨基酶5 亚酸是酸中的一种.真空的降解降解是真空的降解.

更多相关视频

Identification of Post-translational Modifications of Plant Protein Complexes

Identification of Post-translational Modifications of Plant Protein Complexes

Published on: February 22, 2014

Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models

Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models

Published on: October 22, 2012

相关概念视频

Cell Signaling in Plants

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...

Regulation of Transpiration by Stomata

Regulation of Transpiration by Stomata

During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.

Defenses Against Pathogens and Herbivores

Defenses Against Pathogens and Herbivores

Plants present a rich source of nutrients for many organisms, making it a target for herbivores and infectious agents. Plants, though lacking a proper immune system, have developed an array of constitutive and inducible defenses to fend off these attacks.

Plant Cell Wall

Plant Cell Wall

The plant cell wall gives plant cells shape, support, and protection. As a cell matures, its cell wall specializes according to the cell type. For example, the parenchyma cells of leaves possess only a thin, primary cell wall.

IP3/DAG Signaling Pathway

IP3/DAG Signaling Pathway

Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and...