相关实验视频

Updated: Jul 4, 2025

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo

Published on: January 7, 2019

抗瘤免疫疗法使用工程化细菌外膜囊泡与 lysosome-targeting chimeras 融合,由转激素受体介导

Ling-Yan Su¹, Yang Tian¹, Qiang Zheng²

¹College of Food Science and Technology, Yunnan Agricultural University, No. 452 Fengyuan Road, Kunming 650000, China; Yunnan Provincial Key Laboratory of Precision Nutrition and Personalized Food Manufacturing, Yunnan Agricultural University, Kunming 650000, China.

Cell chemical biology

|February 3, 2024

概括

研究人员开发了一种基于细菌外膜囊泡 (OMV) 的新型传递平台 (OMV-LYTAC),用于针对PD-L1等蛋白质的溶酶体降解,在体内显著减少瘤生长.

科学领域:

生物技术是生物技术.
分子生物学分子生物学
免疫治疗是一种免疫疗法.

背景情况:

lysosome-targeting chimeras (LYTACs) 提供一种降解细胞外蛋白的方法,但在设计和体内应用方面面临着挑战.
针对PD-L1等特定蛋白质对于治疗策略至关重要,特别是在癌症免疫治疗中.

研究的目的:

为了设计一种转移素受体 (TfR) 介导的LYTAC (TfR-LYTAC),用于向溶酶体降解.
为TfR-LYTAC开发一种细菌外膜囊泡 (OMV) 输送平台,以增强体内瘤向和疗效.

主要方法:

用TfR-LYTAC进行基因工程,以便通过TfR介导的内细胞化进行内部化.
将TfR-LYTAC与细菌OMV融合在一起,形成OMV-LYTAC.
评估OMV-LYTAC在体内减少瘤生长的疗效及其联合免疫治疗效果.

主要成果:

工程TfR-LYTAC在培养细胞中成功降解PD-L1,但在体内显示出局限性.
OMV-LYTAC平台在体内有效向瘤,并显著减少瘤生长.
OMV-LYTAC显示了PD-1/PD-L1通路抑制,LYTAC介导的降解和免疫激活的综合作用.

结论:

OMV-LYTAC系统提供了一个模块化和有效的策略,用于在体内准和降解细胞外蛋白质.
这种方法为癌症免疫疗法提供了一个有前途的平台,通过将向蛋白质降解与免疫激活相结合.

关键词:

在LYTAC中,我们可以使用LYTAC 有针对性的蛋白质降解降解.癌症免疫疗法免疫疗法奇美拉的交付方式在外膜囊泡中,外膜囊泡

更多相关视频

A Nonviral Approach to Generate Transient Chimeric Antigen Receptor T Cells Using mRNA for Cancer Immunotherapy

A Nonviral Approach to Generate Transient Chimeric Antigen Receptor T Cells Using mRNA for Cancer Immunotherapy

Published on: February 21, 2025

Experimental Melanoma Immunotherapy Model Using Tumor Vaccination with a Hematopoietic Cytokine

Experimental Melanoma Immunotherapy Model Using Tumor Vaccination with a Hematopoietic Cytokine

Published on: February 24, 2023

相关概念视频

Tumor Immunotherapy

Tumor Immunotherapy

Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.

Targeted Cancer Therapies

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...

The Tumor Microenvironment

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...