台式X射线光,以非破坏性的方式量化指甲中的元素含量
Aaron J Specht1, Kolawole E Adesina2, Daniel E Read2
1School of Health Sciences, Purdue University, West Lafayette, IN 47906, United States of America; Harvard T.H. Chan School of Public Health, Boston, MA 02115, United States of America.
The Science of the total environment
|February 3, 2024
概括
桌面X射线光 (XRF) 提供了一种非破坏性的,具有成本效益的方法来测量脚指甲样本中的金属暴露. 这种技术与ICP-MS有很强的相关性,为环境健康研究提供了可行的替代生物标志物.
科学领域:
- 环境健康 环境健康
- 分析化学 分析化学
- 毒理学 毒理学 毒理学
背景情况:
- 金属对全球健康构成重大风险,因此需要可靠的生物标志物来评估暴露.
- 指甲样本比血液具有优势,包括成本效益,收集方便,生物半衰期更长.
- 目前的指甲元素量化,通常使用ICP-MS,可以改进成本和样本保存.
研究的目的:
- 评估桌面X射线光 (XRF) 作为一种非破坏性方法,用于量化人类脚样本中的金属度.
- 为了比较桌面XRF的准确性与标准ICP-MS技术用于脚指甲的元素分析.
- 为了确定桌面XRF在指甲样本中的各种元素的检测极限.
主要方法:
- 桌面X射线光 (XRF) 用于对人类脚指甲样本进行非破坏性基本分析.
- 使用桌面XRF测量 (Pb),铜 (Cu),铁 (Fe) 和 (Se) 的数据与ICP-MS数据进行了比较.
- 计算出了23个额外元素的检测极限.
主要成果:
- 在桌面XRF和ICP-MS之间观察到 (R2=0.92),铜 (R2=0.95), (R2=0.60) 和铁 (R2=0.77) 的强烈相关性.
- 对23种元素的中位数最小检测极限低至0.2μg/g,测量时间为7.5分钟.
- 与先前研究的便携式XRF设备相比,XRF在台式机上显示了较低的检测极限.
结论:
- 桌面XRF是一种高精度和非破坏性的ICP-MS替代品,用于分析脚指甲样本中的金属度.
- 该方法提供了可比的准确性和更好的成本效益,使脚指甲成为首选的生物标志物.
- 基板XRF的增强检测能力支持其在广泛的环境和健康监测中使用.
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