葡萄糖代谢抑制剂GlpR有助于Streptococcus suis的抗氧化压力和毒性
Zijing Liang1, Jiaxuan Lu1, Yinli Bao2
1MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210014, China; Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing 210014, China; WOAH Reference Lab for Swine Streptococcosis, Nanjing 210014, China.
Microbes and infection
|February 3, 2024
概括
瑞杆菌调节器 GlpR 控制糖醇的使用和毒性. 删除GlpR可以促进糖醇的生长,但可以降低抗压力和感染严重程度,从而揭示了它在病变发生中的作用.
科学领域:
- 微生物学 微生物学
- 细菌病原体的产生
- 分子生物学分子生物学
背景情况:
- DeoR家族的转录调节器对细菌生理学至关重要,但它们在链球菌中的作用在很大程度上仍未被描述.
- 葡萄球菌 (Streptococcus suis) 是猪和人类健康的重要病原体,需要对其毒性机制有更深入的了解.
研究的目的:
- 为了识别和表征一种新的DeoR家族调节器,GlpR,在Streptococcus suis.
- 阐明GlpR在糖醇代谢中的功能,氧化应激抵抗力和S. suis.的毒性.
主要方法:
- 基因操纵以创建glpR删除 (ΔglpR) 和补充 (CΔglpR) 菌株.
- 在糖醇介质上分析生长速度.
- 用于基因表达造型的RNA测序 (RNA-seq).
- 用于基因调节分析的β-银酸酶活性测定和电泳性移动性转移测定 (EMSA).
- 评估氧化应激存活率,巨细胞存活率和小鼠毒性.
主要成果:
- GlpR被确定为DNA结合转录调节器,对糖利用至关重要.
- ΔglpR突变体通过抑制甘油代谢基因 (pflB2,pflA1,fsaA) 在甘油介质中表现出增强的生长.
- 在小鼠模型中,ΔglpR突变物在氧化应激下生存率降低,巨细胞的生存率降低,病毒性减弱.
结论:
- GlpR作为S. suis中的糖醇代谢基因抑制剂,影响细菌生长和能源消耗.
- GlpR有助于氧化应激耐药性和毒性,可能通过调节甘油利用率和能量消耗.
- 这项研究增强了对S. suis病变的理解,并扩大了对Streptococci中DeoR家族调节器功能的知识.
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