基于UHPLC-QTOF-MS,根据葡萄品种对中国红葡萄酒进行非有针对性的代谢学认证
Xiao-Li Yin1, Zhi-Xin Peng1, Yuan Pan1
1College of Life Sciences, College of Chemistry and Environmental Engineering, Yangtze University, Jingzhou 434025, China.
Food research international (Ottawa, Ont.)
|February 3, 2024
概括
这项研究开发了一种使用UHPLC-QTOF-MS的代谢学方法来识别中国红葡萄酒品种. 该方法根据其独特的代谢物概况准确区分了卡伯纳索维尼翁,梅洛,卡伯纳格尼什特和皮诺诺瓦尔葡萄酒.
科学领域:
- 分析化学 分析化学
- 食品科学 食品科学 食品科学
- 生物化学 生物化学
背景情况:
- 葡萄酒欺诈,特别是葡萄品种的错误申报,带来了经济挑战.
- 准确的葡萄酒认证对于消费者信任和市场完整性至关重要.
- 代谢学为表征葡萄酒等复杂的生物样本提供了一种强大的方法.
研究的目的:
- 开发和验证一种非向的代谢学方法,用于区分中国红葡萄酒品种.
- 确定与不同葡萄品种相关的关键差异性代谢物和代谢途径.
- 建立一个可靠的分析工具,用于验证红葡萄酒的来源.
主要方法:
- 使用超高性能液态染色学-四极飞行时间质谱法 (UHPLC-QTOF-MS) 进行非目标代谢物分析.
- 分析了来自四个品种的119个中国红葡萄酒样本:卡伯内索维尼翁,梅罗特,卡伯内格尼什特和皮诺诺瓦尔.
- 采用直角局部最小平方区分分析 (OPLS-DA) 和KEGG代谢途径分析来解释数据.
主要成果:
- 在四种红葡萄酒品种中发现了代谢物概况的显著差异.
- 关键的差异性化合物包括黄类,类,类和氨基酸.
- 在外部验证中,OPLS-DA模型获得了高精度 (90.9%的阳性,100%的负离子).
结论:
- 基于UHPLC-QTOF-MS的非向代谢学方法对于识别中国红葡萄酒品种是有效的.
- 印醇和黄类代谢途径与葡萄酒品种特征密切相关.
- 这种方法为葡萄酒认证和打击欺诈行为提供了一个强大的工具.
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