相关实验视频

Updated: Jul 4, 2025

Generation of a Mouse Spontaneous Autoimmune Thyroiditis Model

Generation of a Mouse Spontaneous Autoimmune Thyroiditis Model

Published on: March 17, 2023

在哈西莫托甲状腺炎中使用CRISPR/Cas的治疗潜力:综合性审查

Apoorva Upreti¹, Sayali Mukherjee¹

¹Amity Institute of Biotechnology, Amity University Uttar Pradesh Lucknow Campus, Lucknow 226028, Uttar Pradesh, India.

Current gene therapy

|February 4, 2024

概括

哈西莫托甲状腺炎 (HT) 是一种自身免疫性疾病. 使用CRISPR/Cas的基因编辑显示出通过向动物模型中的候选基因来开发新疗法的前景.

科学领域:

内分泌学在内分泌学.
免疫学免疫学免疫学
遗传学遗传学是一个

背景情况:

哈西莫托甲状腺炎 (HT) 是一种常见的自身免疫内分泌疾病.
它涉及免疫介导的甲状腺组织的破坏,并且具有遗传倾向.
高血压患者患癌症的风险增加,需要新的治疗策略.

研究的目的:

审查与哈西莫托甲状腺炎相关的潜在候选基因.
探索CRISPR/Cas基因编辑在管理和潜在的治疗HT中的应用.
突出CRISPR/Cas生成的动物模型对于理解HT的重要性.

主要方法:

在HT中对候选基因的文献综述.
对针对自身免疫性疾病的CRISPR/Cas基因编辑现有研究进行分析.
对自免疫性甲状腺炎的CRISPR/Cas生成动物模型的检查.

主要成果:

确定了涉及哈西莫托甲状腺炎的潜在候选基因.
注意到关于CRISPR/Cas用于自身免疫性疾病管理的有限但新兴的研究.
突出了使用CRISPR/Cas技术创建的少数动物和人类模型.

结论:

CRISPR/Cas基因编辑为开发HT的新疗法提供了一个有希望的途径.
通过CRISPR/Cas生成的小鼠模型对于阐明HT病理生理学和指导免疫调节策略至关重要.
需要进一步的研究才能充分利用CRISPR/Cas来治疗自身免疫性疾病.

关键词:

这就是CRISPR/CasPR.这就是Cas9的情况.哈西莫托的甲状腺炎.这是一种TSH激素.基因组编辑基因组编辑免疫调节免疫调节免疫调节

更多相关视频

Using CRISPR/Cas9 to Knock Out GM-CSF in CAR-T Cells

Using CRISPR/Cas9 to Knock Out GM-CSF in CAR-T Cells

Published on: July 22, 2019

Electroporation-Based CRISPR-Cas9-Mediated Gene Knockout in THP-1 Cells and Single-Cell Clone Isolation

Electroporation-Based CRISPR-Cas9-Mediated Gene Knockout in THP-1 Cells and Single-Cell Clone Isolation

Published on: February 28, 2025

相关概念视频

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

Homologous Recombination

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...