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一种与塞内卡病毒A VP3蛋白共同表达的复合伪狂犬病病毒的结构和免疫性分析

Qian Tao¹, Lei Xu¹, Yang Zhang¹

¹College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.

Veterinary microbiology

|February 4, 2024

概括

一种新型重组性伪狂犬病病毒 (PRV) 疫苗,rPRV-XJ-ΔTK/gE/gI-VP3,显示出对控制猪中的PRV和塞内卡病毒A (SVA) 的承诺. 这种基于PRV的疫苗提供了保护,并减少了小鼠的疾病严重程度.

科学领域:

兽医病毒学兽医病毒学
猪病研究研究猪病研究
疫苗开发疫苗开发

背景情况:

塞内卡病毒A (SVA) 导致猪异常发病囊泡性疾病 (PIVD),对猪产业构成重大威胁.
伪狂犬病 (PR) 是另一种具有重大经济影响的高度传染性猪病.
对SVA缺乏有效的疫苗,导致广泛爆发.

研究的目的:

开发一种表达SVA VP3蛋白质的复合伪狂犬病病毒 (PRV) 菌株.
评估重组PRV作为潜在的疫苗候选人的安全性和免疫性.
评估其对PRV和SVA挑战的保护有效性.

主要方法:

通过删除TK/gE/gI基因和共同表达VP3蛋白质,构建一个重组PRV菌株 (rPRV-XJ-ΔTK/gE/gI-VP3).
对小鼠疫苗安全性的评估,对PRV的临床症状的监测.
对小鼠进行鼻腔免疫接种,然后对细胞和幽默免疫反应 (针对VP3,gB,PRV和SVA的抗体) 进行评估.
挑战使用毒性PRV-XJ和SVA的研究,以确定保护和观察病理病变.

主要成果:

再组合的PRV成功表达了SVA VP3蛋白,证明了PRV作为病毒载体的适用性.
rPRV-XJ-ΔTK/gE/gI-VP3在小鼠中是安全的,不会引起与PRV相关的临床症状.
鼻腔内接种引发了强大的细胞免疫力和高水平的针对VP3,gB,PRV和SVA的特定抗体.
这种疫苗在感染SVA的小鼠中提供了100%的保护,防止病毒性PRV挑战,并减轻了心脏和肝脏病变.

结论:

再组合PRV菌株rPRV-XJ-ΔTK/gE/gI-VP3是一种安全有效的活病毒载体,用于表达外来蛋白质.
这种复合PRV候选疫苗产生了强大的保护性免疫力,对PRV和SVA.
rPRV-XJ-ΔTK/gE/gI-VP3是控制猪群中PRV变种和SVA的一个有希望的策略.

关键词:

免疫性免疫性免疫性多价值疫苗的使用情况在 PRV 找 PRV.在 SVA VP3 中.

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