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研究免疫细胞中P2X4受体离子通道的方法

Ronald Sluyter1, Tahnee B-D McEwan1, Reece A Sophocleous1

  • 1Molecular Horizons and School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW 2522, Australia.

Journal of immunological methods
|February 4, 2024
PubMed
概括

本综述详细介绍了研究免疫细胞中P2X4受体 (一种联结离子通道) 的方法. 它涵盖了跨物种的基因表达,蛋白质存在和离子通道活性分析.

关键词:
亚丁素5'-三酸盐是一种是一种光技术是一种光技术.在白血球中,白血球结合体带离子通道的离子通道补丁紧方式 补丁紧方式纯原性受体的受体

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Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...
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