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老年人的结膜上皮质中存在衰老细胞的存在

Yasufumi Tomioka¹, Koji Kitazawa^2,3, Kohsaku Numa^1,4

¹Department of Ophthalmology, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Hirokoji-agaru, Kawaramachi-dori, Kamigyo-ku, Kyoto, 602-0841, Japan.

Japanese journal of ophthalmology

|February 4, 2024

概括

细胞衰老,标志着p16INK4a,在老化的人类结膜中增加. 这种积累与减少的干细胞标记物 (Ki67,p63) 和紧结蛋白ZO-1相关.

科学领域:

眼科医生眼科眼科
细胞生物学细胞生物学
衰老研究研究衰老研究

背景情况:

眼睛表面的环境随着年龄的增长而发生变化.
细胞衰老在老化的眼睛表面中的作用仍然在很大程度上未被探索.

研究的目的:

在健康的人类结膜中研究表达p16INK4a的衰老细胞的存在和分布.
为了确定细胞衰老是否与结膜老化有关.

主要方法:

对中老年人和老年人的健康结膜组织样本的分析.
定量实时PCR (RT-qPCR) 用于测量衰老标志物CDKN2A (p16INK4a) 和CDKN1A (p21CIP1/WAF1).
免疫组织化学评估p16INK4a,干细胞标记物 (Ki67,p63) 和紧密结点标记物 (ZO-1).

主要成果:

与中年人相比,老年人显示p16INK4a的RNA和蛋白质表达显著增加.
对于p16INK4a的阳性率在老年人群中显著更高.
在老年结膜组织中观察到Ki67,p63和ZO-1表达的显著减少.

结论:

由p16INK4a积累表明的细胞衰老是人类结膜衰老的一个特征.
衰老细胞的存在与干细胞标记物减少和紧密结合完整性的损害有关.

关键词:

衰老的衰老衰老的衰老细胞衰老细胞衰老结合性结合性结合性结合性 ZO-1 是一个ZO-1 p16INK4a 的使用情况

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