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冷技术中的技术

J Hagedorn¹, G Alkurdi², S-A Barker²

¹Institute for Multiphase Processes, Leibniz University Hannover, Garbsen, 30823, Germany. hagedorn@imp.uni-hannover.de.

|February 5, 2024

概括

冷保存可以阻止生物过程使用低温来保存细胞和组织. 这篇概述详细介绍了冷,储存和解的冷技术,这对细胞治疗和组织工程至关重要.

科学领域:

低温生物学低温生物学
生物技术是生物技术.
生物保护技术生物保护技术

背景情况:

低温保存对于生物材料的长期储存至关重要.
低温停止生化过程,保持结构和功能完整性.
冷保存对于细胞治疗,组织工程和辅助受精至关重要.

研究的目的:

提供关于冷技术的全面概述.
详细介绍生物细胞冷,储存和解的技术.
涵盖冷保护剂,冷成像,微流体和生物银行设备.

主要方法:

审查现有的冷保存技术.
讨论冷保护剂及其机制.
检查冷,储存和解设备和技术.

主要成果:

详细检查冷过程,包括冷保护剂,冷成像,微流体和设备.
专注于解过程和低温保存,用于短期储存.
关于冷技术当前进展的概述.

结论:

冷保存技术对于各种生物医学应用至关重要.
了解冷和解过程是成功长期保存的关键.
这项工作为当前和新兴的冷保存方法提供了洞察力.

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Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...