SARS-Cov-2 小型病毒RNA通过对mRNA 3' UTR的补充结合抑制基因表达
Haley A Delcher1, Jeffrey D DeMeis1, Nicole Ghobar1
1Department of Pharmacology, College of Medicine, University of South Alabama, Mobile, AL.
microPublication biology
|February 5, 2024
概括
SARS-CoV-2 (SC2) 表达小病毒RNA (svRNAs),可以调节宿主免疫基因. 这项研究确定了12个SC2 svRNA,其中一个通过向mRNA 3'未翻译区域来证明具有microRNA类功能.
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
背景情况:
- 由SARS-CoV-2 (SC2) 引起的免疫失调的确切机制尚未完全理解.
- 许多病毒利用小RNA,如microRNA (miRNA),来调节宿主基因表达,特别是与免疫相关的基因.
- 新出现的证据表明,SC2也编码自己的小病毒RNA (svRNAs).
研究的目的:
- 为了识别和描述SC2编码的svRNAs.
- 研究SC2编码的svRNAs在调节宿主基因表达中的功能作用.
- 为了确定SC2 svRNAs是否与人类miRNAs具有相似的功能.
主要方法:
- 生物信息分析用于预测SC2基因组中的潜在svRNAs.
- 反转录定量聚合酶连锁反应 (RT-qPCR) 在SC2感染期间检测和量化表达的svRNA.
- 报告员测试是为了评估已识别的svRNAs通过与mRNA 3'未翻译区域 (3'UTRs) 结合来调节基因表达的能力.
主要成果:
- 鉴定出12种不同的svRNAs在SC2感染期间表达.
- 一个已识别的SC2 svRNA显示了与目标mRNA的3'UTR结合的能力.
- 这种结合事件导致了目标基因表达的调节,模仿了人类miRNAs的机制.
结论:
- SARS-CoV-2编码功能性svRNAs,可以影响宿主基因表达.
- 这些svRNAs代表了一种新的机制,SC2可能会对宿主免疫反应产生失调.
- 对SC2svRNAs的进一步研究可能会揭示用于管理COVID-19的新治疗点.
相关概念视频
02:30siRNA - Small Interfering RNAs
16.8K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
16.8K
01:23RNA Interference
26.0K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.0K
01:22MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
01:23Types of RNA
63.7K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
63.7K
02:15Experimental RNAi
6.1K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.1K
01:56Riboswitches
8.1K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.1K