在泰国东北部的三级医院,从尿液中分离出来的抗万科明素的Enterococcus faecium克隆复合体17的基因组特征
Peechanika Chopjitt1, Parichart Boueroy1, Piroon Jenjaroenpun2
1Faculty of Public Health, Kasetsart University Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon, Thailand.
Frontiers in microbiology
|February 5, 2024
概括
泰国的抗旺素耐药Enterococcus faecium (VREfm) 对多种抗生素表现出耐药性,但仍然对达普素和线索利德敏感. 全基因组测序揭示了携带各种耐药性和毒性基因的CC17菌株,为VREfm流行病学提供了洞察力.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 传染性疾病 传染性疾病
背景情况:
- 抗菌素抗性肠杆菌 (VREs) 是一个重要的全球公共卫生威胁,被世界卫生组织列为高优先级.
- 抗万科素的Enterococcus faecium (VREfm) 是一个特别令人担忧的VRE菌株,经常与医院获得的感染有关.
- 了解VREfm耐药性和毒性的遗传基础对于有效的感染控制至关重要.
研究的目的:
- 调查泰国东北部农村三级医院的VREfm分离物的抗生素敏感性和基因组特征.
- 为了确定这些VREfm菌株中存在的特定抗微生物耐药性基因和毒性因素.
- 为VREfm.m.的当地流行病学提供基因组洞察力.
主要方法:
- 对VREfm分离物进行了抗生素敏感性测试.
- 全基因组测序用于基因组特征.
- 分析包括识别抗微生物耐药性基因和毒性因子.
主要成果:
- 所有的VREfm分离物都表现出对万科米辛,安皮西林,红红素,四环素,西普罗夫洛克萨,诺夫洛克萨和里法的耐药性. 还观察到对尼托黄和tigecycline的高水平耐药性.
- 所有的分离物仍然对氨基醇,达普托米辛和线化物敏感.
- 基因组分析确定所有分离物属于克隆复合体17 (CC17),主要是序列类型 (ST) 80,存在众多抗性基因 (例如,vanA, ermB, tetM) 和毒性基因 (例如,acm, efafm).
结论:
- 来自泰国东北部的VREfm分离物具有多种耐药性和特定的基因组特征.
- CC17,ST80和相关的耐药性/病毒性基因的患病率突出显示了潜在的克隆扩张.
- 这些发现强调了持续监测和基因组研究的重要性,以告知针对VREfm的治疗策略和感染控制措施.
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