模块化工程Rhodotorula toruloides用于α-terpineol的生产
Liting Lyu1,2, Qiongqiong Chen1,3, Haizhao Xue1,3
1Laboratory of Biotechnology, Dalian Institute of Chemical Physics, Dalian, China.
Frontiers in bioengineering and biotechnology
|February 5, 2024
概括
研究人员设计了酵母Rhodotorula toruloides用于α-terpineol的直接生物合成. 这种微生物生产达到1.5mg/L,证明了生产这种有价值的单类醇的新途径.
科学领域:
- 生物技术是生物技术.
- 合成生物学 合成生物学
- 微生物工程 微生物工程
背景情况:
- α-Terpineol 是一种单类酒精,在口味,香味和制药方面具有应用.
- 微生物对α-terpineol的生产仍未得到充分的研究.
- 罗多托鲁拉 (Rhodotorula toruloides) 具有天然的氨酸通路,使其成为生物合成的合适宿主.
研究的目的:
- 为了设计Rhodotorula toruloides用于α-terpineol的直接生物合成.
- 通过模块化工程策略来增强单类产量.
- 确定R. toruloides作为类合成的可行的宿主.
主要方法:
- 增强用于α-terpineol生物合成的前体供应.
- 阻断下游代谢途径以防止产品降解.
- 破坏竞争中的代谢途径以重定向资源.
- 使用模块化策略的R. toruloides的工程.
主要成果:
- 模块化工程策略在α-terpineol生产中产生了不同的改进.
- 改造后的5L6HE5菌株达到1.5mg/L的α-terpineol峰值标位.
- 减少R. toruloides中的脂质积累增强了类合成.
结论:
- Rhodotorula toruloides可以被设计为有效的微生物生产α-terpineol.
- 破坏竞争路径和增加基质供应是有效的策略.
- 这项研究突出了R. toruloides在合成各种单类药物的潜力.
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