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阿佐拉米德通过抑制内细胞网膜应激和抑制氧化应激来改善诱导的细胞毒性

Lingmin Zhang¹, Jianguo Zhang¹, Yingying Zhou¹

¹Department of Laboratory Medicine, Huangyan Hospital, Wenzhou Medical University, Taizhou, Zhejiang Province, People's Republic of China.

|February 5, 2024

概括

阿佐拉米德通过减少内网膜 (ER) 应激和氧化损伤来防止 (Cd) 毒性. 这表明阿佐拉米德可能是中毒的潜在治疗方法.

科学领域:

毒理学毒理学毒理学
细胞生物学细胞生物学
药理学药理学是指药理学的学科.

背景情况:

(Cd) 由于其毒性和持久性,对健康构成重大风险.
亚佐拉米德是一种小分子,向内质网膜 (ER) 应激,但其对Cd毒性的保护作用尚不清楚.

研究的目的:

为了研究阿佐拉米德对诱导的细胞毒性的保护作用.
阐明阿佐拉米德作用的潜在分子机制.

主要方法:

用ICP-MS测量小鼠组织中的Cd积累.
在体外研究中使用了人类细胞系 (HK-2,ARPE-19).
使用各种测试和西式涂抹分析了亡,氧化应激,ER应激,MAPK和自途径.

主要成果:

在小鼠器官中积累的Cd,在肝脏和脏中度最高.
阿佐拉米德通过调节BiP和PERK-eIF2α-CHOP通路来抑制ER压力.
阿佐拉米德降低了氧化应激,减弱了MAPK信号传递 (p38,JNK),抑制了自,并抑制了和视网膜细胞的亡.

结论:

阿佐拉米德表明对诱导的细胞毒性有保护作用.
阿佐拉米德的机制包括缓解ER压力,氧化损伤和亡.
阿佐拉米德作为治疗中毒的治疗剂显示出希望.

关键词:

亚索拉化物 (Azoramide) 是一种亚索拉化物.是的组成部分.细胞内膜网膜应激压力感应合等离子体――质谱学质谱学氧化应激是一种氧化应激.

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