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在患有严重肥胖的青少年中,循环microRNA表达和非酒精性脂肪肝疾病

Yi-Jie Li¹, Brittney O Baumert¹, Nikos Stratakis²

¹Department of Population and Public Health Sciences, Keck School of Medicine, University of Southern California, Los Angeles, CA 90032, United States.

World journal of gastroenterology

|February 5, 2024

概括

血microRNAs (miRNAs) 显示出与严重肥胖的青少年非酒精性脂肪肝疾病 (NAFLD) 特征的显著关联. 这些发现表明特定miRNAs作为NAFLD进展的生物标志物的潜在作用.

科学领域:

儿科肝病学儿科肝病学
分子生物学分子生物学
生物标志物发现发现

背景情况:

非酒精性脂肪性肝病 (NAFLD) 是儿童和青少年常见的慢性肝病.
NAFLD的严重程度从简单的肥胖症到带有炎症和纤维化的非酒精性肥胖肝炎 (NASH) 不等.
在NAFLD中怀疑有改变的循环微RNA (miRNA) 水平,但它们与特定疾病特征的联系尚不清楚.

研究的目的:

研究青少年血miRNA表达和NAFLD组织学特征之间的关系.
测试血miRNA与NAFLD特征相关的假设.

主要方法:

这项研究包括81名患有NAFLD的青少年和54名来自Teen-Longitudinal Assessment of Bariatric Surgery研究的对照.
肝脏活检的特征是NAFLD组织学;血样本进行了miRNA分析 (843个miRNA).
后勤回归分析了miRNAs和NAFLD特征之间的关联,调整共变量;英才途径分析确定了生物功能.

主要成果:

在患有NAFLD的青少年中发现了16个上调和22个下调的血miRNA.
在miRNAs和NASH (52个miRNA),纤维化 (16个miRNA),气球运动 (15个miRNA) 和叶状炎症 (9个miRNA) 之间发现了显著的关联.
包括miR-411-5p,miR-122-5p和miR-193a-5p在内的特定miRNA与多种组织学特征一致相关,其中一些与瘤进展和病毒性肝炎途径有关.

结论:

血miRNA与严重肥胖的青少年的NAFLD组织学特征有关.
需要对更大,更多样化的队伍进行进一步的研究,以验证miRNAs作为潜在的NAFLD生物标志物.

关键词:

气球退行症是什么?肝脏纤维化肝脏纤维化叶片炎症叶片炎症这是一个微型RNA.非酒精性脂肪性肝炎的疾病.非酒精性脂肪性肝病是非酒精性脂肪性肝病.

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...