佐 (a) 二烯和2,2',4,4'-四博乙烯对大鼠甲状腺暴露的影响 通过减弱总反射里埃转换红外光谱法
QiuFeng Lao1,2,3, LiJun Yang1,2, ShuZhen Liu1,2
1Guangxi Key Laboratory of Environmental Exposomics and Entire Lifecycle Heath, Guilin Medical University, Guilin, Guangxi 541199, China.
广泛传播的污染物甲 (B[a]P) 和BDE-47损害了老鼠的甲状腺,改变了脂质,蛋白质和碳水化合物结构. 里埃变换红外光谱和SVM准确地确定了这些有毒效应.
科学领域:
- 环境毒理学环境毒理学
- 生物化学 生物化学
- 频谱学是一种光谱学.
背景情况:
- 甲 (B[a]P) 和2,2',4,4'-四甲乙烯 (BDE-47) 是普遍存在的环境污染物.
- 这些化合物已知会破坏甲状腺功能.
研究的目的:
- 评估B[a]P和BDE-47.7暴露后大鼠甲状腺组织的生物化学变化.
- 为了评估弱总反射的功效,富里埃变换红外光谱学 (ATR-FTIR) 与支持矢量机器 (SVM) 结合检测这些变化.
主要方法:
- 鼠被暴露在B[a]P和BDE-47.7中.
- 用ATR-FTIR光谱学分析了甲状腺组织.
- 支持矢量机 (SVM) 算法用于数据分类.
主要成果:
- 暴露于B[a]P和BDE-47导致显著的甲状腺毛囊破坏和上皮细胞死亡.
- ATR-FTIR确定了与脂质 (C=O延伸),蛋白质 (胺I和II),碳水化合物 (C-OH,C-O,C-C延伸) 和原蛋白 (基延伸) 相关的特定生化变化.
- 在将对照组与暴露组区分和在不同暴露组之间区分方面,SVM实现了高准确性 (96%),高准确性 (93%).
结论:
- B[a]P和BDE-47诱导了大鼠甲状腺组织的形态和生化损伤.
- 与SVM相结合的ATR-FTIR光谱是一种强大的工具,用于识别和分类环境污染物对甲状腺组织的有毒影响.
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