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两种方法对二甲基氨酸的量化方法的比较.

Vendula Sudová1,2, Pavel Prokop1,2, Ladislav Trefil1,2

  • 1Biomedical Center, Faculty of Medicine in Pilsen, Charles University, Alej Svobody 1655/76, Pilsen, 32300, Czech Republic.

Practical laboratory medicine
|February 5, 2024
PubMed
概括

这项研究验证了一种液体染色学-并联质谱法 (LC-MS/MS) 方法,用于测量慢性病 (CKD) 中的二甲基氨酸. 免疫试验高估了ADMA和低估了SDMA,这表明简单的样本稀释可以提高准确性.

关键词:
不对称的二甲基亚尔基尼尼.在CKD中,它是最好的.艾丽莎·伊丽莎·伊丽莎这是LC-MS/MS.对称的二甲基亚尔吉尼因.

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科学领域:

  • 生物化学 生物化学
  • 分析化学 分析化学
  • 临床化学 临床化学

背景情况:

  • 双甲基氨酸 (ADMA和SDMA) 是与慢性病 (CKD) 相关的生物标志物.
  • 准确测量这些生物标志物对于患者管理和研究至关重要.
  • 与先进的分析技术相比,现有的免疫测试方法的准确性可能有局限性.

研究的目的:

  • 验证已公布的液体染色学-并联质谱法 (LC-MS/MS) 方法,用于量化不对称二甲基氨酸 (ADMA) 和对称二甲基氨酸 (SDMA).
  • 将LC-MS/MS获得的结果与传统免疫试验的结果进行比较.
  • 评估这两种方法的准确性和可靠性,在健康个体和患有不同阶段的CKD的患者中进行.

主要方法:

  • 在Dionex UltiMate 3000系统上使用UHPLC-MS/MS进行分析,并配备Bruker amaZon SL离子陷.
  • 方法比较涉及通过巴布洛克回归和布兰德·奥尔特曼图.
  • 验证和比较使用来自40名健康志愿者和40名患有CKD的患者的样本进行.

主要成果:

  • LC-MS/MS和免疫测试显示了ADMA (斯皮尔曼R=0.858) 和SDMA (斯皮尔曼R=0.895) 两者的强烈相关性.
  • 对ADMA的免疫检测结果被高估了大约30%,而SDMA水平被低估了大约3%.
  • 慢性病患者的平均ADMA度为0.82±0.16μmol/L (LC-MS/MS) 与1.06±0.30μmol/L (免疫测试) 相比.
  • 慢性病患者的SDMA平均水平为2.14 ± 0.88μmol/L (LC-MS/MS) 与1.65 ± 0.52μmol/L (免疫测试) 相比.

结论:

  • LC-MS/MS方法为测量ADMA和SDMA提供了经过验证和可靠的方法.
  • 在LC-MS/MS和免疫检测结果之间存在显著的差异,特别是在ADMA.
  • 简单的样品稀释可能会提高对二甲基氨酸的免疫测试测量的准确性.