从昆残留物中生产生物,使用暗发酵,并估计其微生物多样性
1Ardahan University, Department of Environmental Health, Ardahan, Turkey.
Heliyon
|February 5, 2024
概括
这项研究探讨了使用暗发酵从花废物中生产生物 (bio-H2) 的方法. 研究人员发现了显著的生物产量,确定了参与该过程的关键细菌物种.
科学领域:
- 生物技术和生物工程 生物技术和生物工程
- 可再生能源可再生能源来源
- 环境科学 环境科学
背景情况:
- 农业残留物是丰富的,低成本的,富含碳水化合物的材料,适合生物 (生物-H2) 生产.
- 暗发酵是一种有效的生物方法,用于将废物转化为生物.
- 昆残留物通过暗发酵产生生物的潜力以前没有被研究过.
研究的目的:
- 通过暗发酵来研究从花残留物中生产生物的潜力.
- 优化操作条件,包括基质类型和pH值,以获得最大的生物产量.
- 确定主导的微生物群落及其在发酵过程中的作用.
主要方法:
- 无氧批量生物反应器在36°C以热预处理的无氧混合细菌运行.
- 基质包括原始菜残留物和菜提取液,其度和pH值各不相同.
- 进行了气体分析,分类内容分析和有机酸分析,以评估生物的生产和微生物组成.
主要成果:
- 在所有测试的生物反应器中检测到生物的产生.
- 在pH值为4.0的情况下,观察到最大的生物H2产量,含有1.00 g/L原始昆残留物 (61,537.10^-4 mL).
- 确定的主要细菌是 *Clostridium butyricum* 和 *Hathewaya histolytica* 在原始菜反应堆中,以及 *Fonticella tunisiensis* 在菜提取物反应堆中.
结论:
- 麦残留物是通过暗发酵生产生物的可行基质.
- 优化条件,特别是pH值和基质度,显著影响生物产量.
- 了解微生物生态对于提高从农业废物制造生物的生产至关重要.
相关概念视频
01:23Microbial Fermentation
1.8K
Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
1.8K
01:26Microbes and Methanogenesis
91
Methanogenesis is a critical microbial process in anaerobic ecosystems responsible for the biological production of methane, a potent greenhouse gas and valuable biofuel. This metabolic pathway is primarily facilitated by methanogenic archaea, which thrive in anoxic environments such as wetlands, sediments, and animal gastrointestinal tracts. The absence of oxygen in these habitats prevents aerobic respiration, thereby favoring alternative biochemical pathways for organic matter degradation.In...
91
01:26Microbial Bioremediation of Hydrocarbons
150
Bioremediation is an environmentally sustainable process that employs living organisms—primarily microorganisms—to degrade or neutralize pollutants from contaminated environments. In oil spills and hydrocarbon pollution, bioremediation involves the use of hydrocarbon-degrading bacteria to transform toxic compounds into less harmful substances. This approach leverages natural microbial metabolic processes and is considered both cost-effective and ecologically favorable compared to...
150
01:29Microbes in Food Production
403
Microbial fermentation is central to food biotechnology, enhancing flavor, texture, preservation, and stability. Fermentative microorganisms metabolize carbohydrates into organic acids, alcohols, and other metabolites that inhibit spoilage organisms and improve digestibility while contributing distinctive sensory qualities.In baking, amylases naturally present in flour hydrolyze starch into monosaccharides such as glucose, which Saccharomyces cerevisiae ferments anaerobically. Through...
403
01:27Microbes in the Production of Fermented Foods
327
Lactic acid bacteria (LAB) and molds are instrumental in fermenting plant-based foods to enhance preservation and ensure year-round availability. These microbial processes convert plant carbohydrates into organic acids and other metabolites that inhibit spoilage organisms and contribute to the sensory qualities of the final product.In sauerkraut production, cabbage goes through a microbial succession that starts with cocci such as Leuconostoc mesenteroides. These microbes begin fermentation by...
327
01:25Production of Organic Acids
105
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
105