含有BTB/TAZ域的蛋白质CmBT1-介导的CmANR1泛化对花的根部发育产生负面调节
Lian-Da Du1, Zhang-Ji Guan1, Yan-Hong Liu1
1Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, College of Horticulture Science and Engineering, Shandong Agricultural University, Taian, 271018, China.
一种新型蛋白质CmBT1通过准ANR1基因来负面调节花根的发育. CmBT1通过ubiquitination促进ANR1蛋白降解,最终抑制根生长.
科学领域:
- 植物生物学 植物生物学
- 分子植物生理学分子植物生理学
- 遗传学 是一个遗传学.
背景情况:
- 根系的发展对于植物的适应至关重要.
- MADS盒子基因ANR1积极调节香的根部发育.
- CmANR1的上游调节者在很大程度上是未知的.
研究的目的:
- 为了确定CmANR1.1.的上游调节者.
- 阐明调节CmANR1功能的分子机制.
- 了解CmBT1在花根发育中的作用.
主要方法:
- 酵母-两个混合 (Y2H) 查查.
- 双分子光补充 (BiFC) 方法
- 拉下测试试剂的测试方法
- 在体外和体内生物功能测定.
- 立方体化试验测定
- 定量PCR是一种定量PCR.
主要成果:
- 确定CmBT1是CmANR1的交互伙伴.
- CmBT1 负面调节花的根部发育.
- 通过ubiquitin/26S蛋白酶体通路,CmBT1调解了CmANR1的全域化和降解.
- CmBT1降低了CmPIN2转录,这是一个对根部发育至关重要的基因.
结论:
- CmBT1 作为花根发育的负调节剂.
- 通过ubiquitination,CmBT1降解了CmANR1蛋白,从而抑制了根的生长.
- 这项研究揭示了一种新的调节途径,涉及CmBT1-介导的CmANR1.1降解.
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