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在两个心肌细胞模型中,fludioxonil通过线粒体功能障碍和氧化应激诱导心脏毒性
Su-Min Seong1, Ryeo-Eun Go1, Hong Kyu Lee1
1Laboratory of Biochemistry and Immunology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea.
Environmental toxicology
|February 5, 2024
概括
化剂暴露会通过破坏线粒体,增加氧化应激,并触发亡来损害心脏细胞. 这种杀菌剂毒性对心血管健康构成风险,需要谨慎的农业使用.
科学领域:
- 心血管毒理学心血管毒理学
- 线粒体生物学 线粒体生物学
- 环境健康 环境健康
背景情况:
- 弗卢迪尼 (Flu) 是一种广泛使用的甲杀菌剂,具有已知的内分泌干扰性.
- 以前的研究表明,流感会诱导各种细胞系的亡和病理变化.
- 弗卢迪奥尼尔对心肌细胞的影响在很大程度上仍未得到研究.
研究的目的:
- 调查Fludioxonil对人类 (AC16) 和老鼠 (H9c2) 心肌细胞中的线粒体的影响.
- 为了确定Fludioxonil是否会诱导心脏细胞的亡和线粒体功能障碍.
主要方法:
- 使用水溶性四试验评估细胞活力.
- 通过Annexin V/propidium iodide染色和对亲细胞亡标记物的分析 (Bax,分裂-caspase3) 评估的细胞亡.
- 通过使用JC-1染色测量氧消耗率 (OCR) 和线粒体膜潜力 (MMP),以及反应性氧物种 (ROS) 生产来评估线粒体功能.
主要成果:
- 弗卢迪尼尔降低了细胞活力,并诱导了暗的形态变化.
- 增加的Annexin V阳性细胞和升高的Bax和分裂-caspase 3表达证实了亡.
- 弗卢迪尼尔降低了OCR和MMP,同时增加了线粒体ROS的产生,表明了显著的线粒体失调.
结论:
- 弗卢迪尼尔会诱导心肌细胞中的线粒体功能障碍和氧化应激,导致细胞亡.
- 这种杀菌剂对心血管疾病构成潜在风险.
- 由于可能对心血管系统产生不良健康影响,Fludioxonil的农业用途需要谨慎使用.
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