硫法调节巨细胞M1/M2极化,以减轻巨细胞诱导的Caco-2细胞损伤在炎症环境中
Ting Yi1, Zhiyin Liu2, Haokun Jia1
1Department of Hematology, The Affiliated Changsha Central Hospital, Hengyang Medical School, University of South China, Changsha, Hunan, China.
Iranian journal of immunology : IJI
|February 5, 2024
概括
硫法 (SFN) 将巨细胞的两极分化从M1转移到M2,有可能治疗炎症性肠病 (IBD). 这通过Nrf2激活发生,减少炎症标志物并改善肠道屏障功能.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 胃肠病学 胃肠病学
背景情况:
- 大细胞极化失衡 (M1/M2) 是炎症性肠病 (IBD) 发病的关键.
- 硫黄 (SFN) 在治疗炎症疾病方面表现有前途.
研究的目的:
- 为了确定SFN对巨细胞极化的影响.
- 阐明SFN行动的监管机制.
主要方法:
- 鼠标骨髓衍生巨细胞 (BMDMs) 被偏向于M1或M2表型.
- 应用了SFN治疗和Nrf2抑制.
- 通过流细胞测量,qRT-PCR和西部斑点来评估巨细胞极化.
- 评估了细胞功能和肠道屏障完整性.
主要成果:
- SFN (最佳10μM) 促进了M2极化,并抑制了M1标记物 (IL-12,IL-6,TNF-α).
- SFN增加了M2标记物 (YM1,Fizz1,Arg1,CD206+) 和Nrf2通路的激活 (Nrf2,NQO1,HO-1).
- Nrf2抑制逆转了SFN的极化作用;SFN部分保护Caco-2细胞免受LPS诱导的损伤.
结论:
- SFN 作为 Nrf2 激动剂,促进 M2 巨细胞两极分化.
- 通过调节巨细胞平衡和保护肠道屏障,SFN显示出作为IBD治疗剂的潜力.
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