抗氧化应激诱导的破坏耳细胞是由盲目的抗氧化剂治疗引起的
Liling Li1, Dan Chen1, Xuexin Lin1
1Department of Otolaryngology-Head and Neck Surgery, The Third Affiliated Hospital of Sun Yat-Sen University, Guang Zhou, Guangdong, China.
概括
过度使用N-乙半氨酸 (NAC) 等抗氧化剂可以导致抗氧化应激,损害耳细胞. 高度的M40403和辅酶Q10也会导致毛细胞脱落,这表明不适当的抗氧化剂使用会损害听力.
科学领域:
- 听力科学 听力科学
- 细胞生物学 细胞生物学
- 毒理学 毒理学 毒理学
背景情况:
- 抗氧化剂对于细胞保护至关重要.
- 然而,过度或不适当使用可能会引起不良影响.
- 了解抗氧化剂对耳细胞的剂量依赖影响至关重要.
研究的目的:
- 调查过度抗氧化剂使用可能导致耳细胞损伤的可能性.
- 为了验证高度的抗氧化剂是否会导致抗氧化应激,并加剧耳损伤.
主要方法:
- 利用HEI-OC1细胞评估N-乙半氨酸 (NAC) 对细胞密度,活力和细胞亡的影响.
- 在Hoechst,细胞计数套件-8和Annexin V/PI染色中工作.
- 在用M40403和辅酶Q10治疗的耳扩展器中使用激活的caspase-3标记分析了亡.
主要成果:
- 增加NAC度 (高达1000μmol/L) 显著降低了HEI-OC1细胞密度和活力.
- 较高的NAC水平在HEI-OC1细胞中诱导了亡.
- M40403和辅酶Q10在度>30μmol/L时会导致剂量依赖的耳毛细胞损伤和亡.
结论:
- 抗氧化剂的不适当应用可能会对耳毛细胞造成严重损伤.
- 这项研究强调了均衡和适当的抗氧化剂管理的重要性.
- 研究结果表明,超过抗氧化剂对耳健康有害的值.
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