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在牛Rhipicephalus microplus中,抗化剂和乙胆酶基因多态性之间的关系

Raquel Cossio-Bayugar¹, Francisco Martinez-Ibañez², Hugo Aguilar-Diaz¹

¹Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias INIFAP, Boulevard Cuauhnahuac 8534, Jiutepec, Morelos, CP 625574, México.

Parasite (Paris, France)

|February 5, 2024

概括

在Rhipicephalus microplus中,抗酸耐药性与较高的乙胆酶 (AChE2) 表达和关键氨基酸变化有关. 这些AChE2酶的变化影响了糖结合,解释了耐药性机制.

科学领域:

兽医昆虫学兽医昆虫学
分子生物学分子生物学
生物化学生物化学

背景情况:

在Rhipicephalus microplus中,抗化剂的耐药性对牲畜健康和农业生产率构成重大威胁.
了解阻力背后的分子机制对于开发有效的控制策略至关重要.

研究的目的:

为了识别与Rhipicephalus microplus.acaricide耐药性相关的乙胆酶转录2 (AChE2) 中的氨基酸多态性.
通过分子建模,研究这些多形态对乙化物-蛋白相互作用的影响.

主要方法:

定量PCR (qPCR) 用于评估耐药和易受的ACHE2基因表达水平.
氨基酸测序用于识别耐药和敏感菌株之间的ACHE2变异.
3D分子对接,以建模乙酸和ACHE2催化部位之间的相互作用.

主要成果:

与敏感的相比,耐酸的Rhipicephalus microplus显示出明显更高的ACHE2表达.
在耐药的ACHE2中发现了9种基性氨基酸替代.
对接模型表明,这些氨基酸多态性改变了酸结合口袋配置.

结论:

在ACHE2中的氨基酸多态性是导致Rhipicephalus microplus.acaricide耐药性的关键因素.
由于这些多形态,ACHE2催化部位的改变相互作用解释了抵抗现象.
这项研究为有针对性的策略提供了基础,以克服的抗素抵抗.

关键词:

牛的是牛的.抗杀虫剂耐药性抗杀虫剂耐药性是一种蛋白质的3D建模.这是RT-PCR.

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